Detailed information of TA system TA140820

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44053..44317
• Replicon: plasmid pNMBU-W10C18_02
• Accession: NZ_CP044402
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: F7O57_RS00300
• Protein ID: WP_001387489.1
• Coordinates: 44053..44205 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	44257..44317 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS00265	39145..39678	-	534	WP_001221666.1	lipocalin family protein	-
F7O57_RS00270	39772..40917	-	1146	WP_039023136.1	class C extended-spectrum beta-lactamase CMY-42	-
F7O57_RS00275	41370..42067	-	698	Protein_54	IS1 family transposase	-
F7O57_RS00280	42292..42501	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
F7O57_RS00285	42893..43069	+	177	WP_001054897.1	hypothetical protein	-
F7O57_RS00290	43134..43430	-	297	WP_011264046.1	hypothetical protein	-
F7O57_RS00295	43730..43981	+	252	WP_001291964.1	hypothetical protein	-
F7O57_RS00300	44053..44205	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	44257..44317	+	61	NuclAT_0	-	Antitoxin
-	44257..44317	+	61	NuclAT_0	-	Antitoxin
-	44257..44317	+	61	NuclAT_0	-	Antitoxin
-	44257..44317	+	61	NuclAT_0	-	Antitoxin
F7O57_RS00305	44520..44867	+	348	Protein_60	protein finQ	-
F7O57_RS00310	45053..46210	+	1158	Protein_61	IS1380-like element ISEc9 family transposase	-
F7O57_RS00315	46266..46963	+	698	Protein_62	IS1 family transposase	-
F7O57_RS00320	46977..47282	-	306	Protein_63	transcription termination factor NusG	-
F7O57_RS00325	47724..48011	-	288	WP_000074855.1	conjugal transfer protein TraA	-
F7O57_RS00330	47929..48192	-	264	WP_032180564.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..49118	49118
	-	inside	IScluster/Tn	blaCMY-42	-	39772..46541	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T140820 WP_001387489.1 NZ_CP044402:c44205-44053 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T140820 NZ_CP059467:c3797776-3797510 [Aromatoleum bremense]
GTGGTAAGTTGGGAAATCGTTTATGCCAGGCAAGCACTGAAGGATGCAAAGAAACTTGCTGCAAGCGGTCTTAAACCAAA
GGCGCAAGAAGTGCTCGTGGTTCTTGCCGACGACCCGTTTAAAAATCCACCTCCCTTTGAAAAATTGGTCGGTGATCTTG
CCGGCGCATACTCACGTCGCATCAATATTCAACACCGCATCGTGTACGAAGTCTTTGCCAACGAGAAAACGGTTCGCGTC
CTGCGTATGTGGACGCACTATGAATGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT140820 NZ_CP044402:44257-44317 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin