Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 1667602..1668166 Replicon chromosome
Accession NZ_CP044361
Organism Lacticaseibacillus paracasei strain TD 062

Toxin (Protein)


Gene name yafQ Uniprot ID K6S2P8
Locus tag F5J09_RS08210 Protein ID WP_003602097.1
Coordinates 1667602..1667904 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag F5J09_RS08215 Protein ID WP_003570051.1
Coordinates 1667897..1668166 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F5J09_RS08200 1663897..1666074 - 2178 WP_159301214.1 RNA-binding transcriptional accessory protein -
F5J09_RS08205 1666310..1667002 - 693 Protein_1561 hypothetical protein -
F5J09_RS14720 1667120..1667263 - 144 WP_191994355.1 hypothetical protein -
F5J09_RS08210 1667602..1667904 - 303 WP_003602097.1 type II toxin-antitoxin system YafQ family toxin Toxin
F5J09_RS08215 1667897..1668166 - 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
F5J09_RS08220 1668540..1669208 - 669 WP_159301215.1 GNAT family N-acetyltransferase -
F5J09_RS08225 1669322..1670302 + 981 Protein_1566 IS5-like element ISLrh3 family transposase -
F5J09_RS08230 1670325..1671245 - 921 WP_159301216.1 IS30 family transposase -
F5J09_RS08235 1671355..1671795 + 441 Protein_1568 transposase -
F5J09_RS08240 1671863..1672492 - 630 WP_159301323.1 DUF2399 domain-containing protein -
F5J09_RS08245 1672555..1672881 - 327 WP_003658351.1 YrdB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-98)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11442.21 Da        Isoelectric Point: 9.9697

>T140812 WP_003602097.1 NZ_CP044361:c1667904-1667602 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLGK

Download         Length: 303 bp

>T140812 NZ_CP059467:1331721-1332104 [Aromatoleum bremense]
ATGAAGGGTGTCCTGGTCGACACATCGGTATGGGTTGACCACTTCCGACAACGCAATGATGTTCTGGTGGACTTGCTCGA
TTTGGACCTGGCGCTGACGCATCCAATGGTCCTGGTCGAACTTGCCTGCGGAACACCACCCGCACCGCGAGTTCGGACAC
TCAGCGACATAGGGCTCCTTCAACAGACGCAACAGGCGAGTTTGCGCGAGGTGATGGACTTCATTGAACGCGAAAAACTG
TATGGGCTTGGGTGCGGGCTGGTTGACATGGTTTTGTTGGCGTCAACGCTGATCACACCGGGCGCAGAGCTGTGGACACT
GGACAAGCGCTTGGTCGCGCTGTCAGAACGGTTCGGCGTGATGCATCGGTCGATCTTGCAGTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT140812 WP_003570051.1 NZ_CP044361:c1668166-1667897 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT140812 NZ_CP059467:1331521-1331724 [Aromatoleum bremense]
ATGAGAACGACTGTCACGATCGATGATGATCTGTACGAAAAGGCCCTGGAAGTGGCCGATCCGGCTATGGACAGGGCCGA
TCTCTTCCGCGAGGCGATGAAAACTTTCGTGCGAGTACAAGCCGCCAAGCGCCTTGCTGCGTTGGGGGGCACCATGCCGG
AGATGCAGGATGTGCCGCGACGGCGCGGCGAGCCCTCGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S2P8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References