Detailed information of TA system TA140809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 11574..12159
• Replicon: plasmid pCAM180B
• Accession: NZ_CP044358
• Organism: Acinetobacter baumannii strain CAM180-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ABCAM1_RS19360
• Protein ID: WP_017725647.1
• Coordinates: 11839..12159 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ABCAM1_RS19355
• Protein ID: WP_000369780.1
• Coordinates: 11574..11846 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABCAM1_RS19330	7191..7544	-	354	WP_004282120.1	hypothetical protein	-
ABCAM1_RS19335	7851..8192	+	342	WP_039902700.1	hypothetical protein	-
ABCAM1_RS19340	8770..9726	+	957	WP_004282117.1	replication initiation protein RepM	-
ABCAM1_RS19345	9719..10273	+	555	WP_004282116.1	helix-turn-helix domain-containing protein	-
ABCAM1_RS19520	10294..10437	+	144	WP_171054347.1	hypothetical protein	-
ABCAM1_RS19350	10782..11099	+	318	WP_032023430.1	hypothetical protein	-
ABCAM1_RS19355	11574..11846	-	273	WP_000369780.1	helix-turn-helix domain-containing protein	Antitoxin
ABCAM1_RS19360	11839..12159	-	321	WP_017725647.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ABCAM1_RS19365	12345..12542	-	198	WP_000476222.1	hypothetical protein	-
ABCAM1_RS19370	12609..12836	-	228	WP_000921865.1	hypothetical protein	-
ABCAM1_RS19375	12933..13148	-	216	WP_000071892.1	cold-shock protein	-
ABCAM1_RS19380	13474..13905	-	432	WP_000390052.1	hypothetical protein	-
ABCAM1_RS19385	13923..14189	-	267	WP_006581723.1	hypothetical protein	-
ABCAM1_RS19390	14399..15835	+	1437	WP_108496623.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..16096	16096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (17-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12237.02 Da
• Isoelectric Point: 7.9849

>T140809 WP_017725647.1 NZ_CP044358:c12159-11839 [Acinetobacter baumannii]
MLFIETSIFTRQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T140809 NZ_CP059457:200905-201255 [Priestia megaterium]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10122.63 Da
• Isoelectric Point: 8.6582

>AT140809 WP_000369780.1 NZ_CP044358:c11846-11574 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPTLI

Nucleotide

• Length: 273 bp

>AT140809 NZ_CP059457:200619-200900 [Priestia megaterium]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA