Detailed information of TA system TA140808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 66246..66708
• Replicon: plasmid pCAM180A
• Accession: NZ_CP044357
• Organism: Acinetobacter baumannii strain CAM180-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S3T2E4
• Locus tag: ABCAM1_RS19145
• Protein ID: WP_016653392.1
• Coordinates: 66246..66434 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ABCAM1_RS19150
• Protein ID: WP_001258992.1
• Coordinates: 66421..66708 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABCAM1_RS19105	61907..62263	+	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ABCAM1_RS19110	62256..62558	+	303	WP_001140619.1	XRE family transcriptional regulator	-
ABCAM1_RS19115	62551..62760	+	210	WP_000069471.1	hypothetical protein	-
ABCAM1_RS19120	62885..63097	-	213	WP_016653394.1	hypothetical protein	-
ABCAM1_RS19130	63856..64365	-	510	Protein_60	IS5 family transposase	-
ABCAM1_RS19135	64430..65595	+	1166	WP_085940844.1	IS3-like element ISAba22 family transposase	-
ABCAM1_RS19140	65649..65969	+	321	WP_016653393.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ABCAM1_RS19145	66246..66434	+	189	WP_016653392.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ABCAM1_RS19150	66421..66708	+	288	WP_001258992.1	helix-turn-helix transcriptional regulator	Antitoxin
ABCAM1_RS19155	66992..67216	-	225	WP_016653391.1	hypothetical protein	-
ABCAM1_RS19160	67240..67797	-	558	Protein_66	hypothetical protein	-
ABCAM1_RS19165	67837..68856	-	1020	Protein_67	terminase	-
ABCAM1_RS19170	68929..70137	+	1209	WP_021511398.1	IS256 family transposase	-
ABCAM1_RS19175	70177..70476	-	300	Protein_69	terminase	-
ABCAM1_RS19180	70460..71001	-	542	Protein_70	DUF2280 domain-containing protein	-
ABCAM1_RS19185	71061..71702	-	642	WP_021511117.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92034	92034
	-	inside	IScluster/Tn	-	-	47044..76116	29072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-55)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-90)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7183.65 Da
• Isoelectric Point: 10.7295

>T140808 WP_016653392.1 NZ_CP044357:66246-66434 [Acinetobacter baumannii]
MSKGLFELRLKSQECIARVMYCTLVGKRIVMLHSFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 189 bp

>T140808 NZ_CP059449:c444806-444603 [Azospirillum brasilense]
ATGGTCACGAGCGCTGCGCACAGCGCGTGGCCTTCGGATGTTGAACTGACCGCTTATGCCGAGGCTGGTCTGAAGAAATC
CTGCAAGTTCCGCTGCAAGCTTTTTACGCTTCAGGATGATCTGGTGTTCTATGGACTCGGCATGCTGGCTGAGGCCGATC
GGCTGGCCGTGACCGCGGTGACCTCTGGACTTCTGCCGCGATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10563.32 Da
• Isoelectric Point: 9.7308

>AT140808 WP_001258992.1 NZ_CP044357:66421-66708 [Acinetobacter baumannii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTALREAGLSQAQIAERMGTNVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT140808 NZ_CP059449:c445151-444927 [Azospirillum brasilense]
ATGCACATCTCGCGGCTGGCCGCCGACAACAGGGCGTTCATTTCCCAGGACGTCCGCGACGCCTTGGGCTTGAAGGAAGG
CGACCCGATCCTGTTTTCCGTGAAGGACGGGCAGGCGGTGATCCGCAAGGCGCCCATCGTGGATACCGCTTATCTGAAAG
GGCTGGAAGGCGGTCTGTCGGAATGGGCCTTGCCCGAGGACGACGAGGCGTTTGATGATCTTTGA

Structures

Toxin

• AlphaFold DB: S3T2E4