Detailed information of TA system TA140807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 61907..62558
• Replicon: plasmid pCAM180A
• Accession: NZ_CP044357
• Organism: Acinetobacter baumannii strain CAM180-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S3SZA7
• Locus tag: ABCAM1_RS19105
• Protein ID: WP_000269903.1
• Coordinates: 61907..62263 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ABCAM1_RS19110
• Protein ID: WP_001140619.1
• Coordinates: 62256..62558 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABCAM1_RS19075	57807..58088	-	282	WP_006582667.1	putative addiction module antidote protein	-
ABCAM1_RS19080	58089..58391	-	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ABCAM1_RS19085	58591..59253	+	663	WP_016653395.1	hypothetical protein	-
ABCAM1_RS19105	61907..62263	+	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ABCAM1_RS19110	62256..62558	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
ABCAM1_RS19115	62551..62760	+	210	WP_000069471.1	hypothetical protein	-
ABCAM1_RS19120	62885..63097	-	213	WP_016653394.1	hypothetical protein	-
ABCAM1_RS19130	63856..64365	-	510	Protein_60	IS5 family transposase	-
ABCAM1_RS19135	64430..65595	+	1166	WP_085940844.1	IS3-like element ISAba22 family transposase	-
ABCAM1_RS19140	65649..65969	+	321	WP_016653393.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ABCAM1_RS19145	66246..66434	+	189	WP_016653392.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ABCAM1_RS19150	66421..66708	+	288	WP_001258992.1	helix-turn-helix transcriptional regulator	-
ABCAM1_RS19155	66992..67216	-	225	WP_016653391.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92034	92034
	-	inside	IScluster/Tn	-	-	47044..76116	29072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13486.45 Da
• Isoelectric Point: 7.1645

>T140807 WP_000269903.1 NZ_CP044357:61907-62263 [Acinetobacter baumannii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T140807 NZ_CP059448:3685047-3685298 [Pseudomonas fluorescens]
GTGTCAAAAAATGAAAAGCTGCTCGCCAAACTGCTCAACGAGCACATGGCCTTTACCTGGCCAGAGCTCGTAACGCTGCT
GCATCGCTTGGGCTACACACAGATTGAAGGGGCTGGAAGCCGCGTCAAGTTCGACATCGGCGACCCCAGTGCAATGATCA
GCTTGCACAAGCCTCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAGATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT140807 WP_001140619.1 NZ_CP044357:62256-62558 [Acinetobacter baumannii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT140807 NZ_CP059448:3685295-3685660 [Pseudomonas fluorescens]
GTGAACAACCAACTGAAATACAACGGCTACATCGGCTCTATCGAAGCCAGCCTCGAAGACAACTGCCTGTTCGGCAAGAT
TCTGTTTATCAAGGCGCTGGTCAGTTACGAAGGGAAAACCGTCGCAGAGTTGGACGCTGCGTTCCGTGAGGCCGTCGATG
ACTACCTCGCGACATGCCACGCGCTGGGGCAAACGCCAGAGAAGCCTTGCAAGGGCTCATTCAACGTACGGGTCGGCCAT
GACCTGCACTTGGCAGCGGCACTGGCGGCCACACGTAAAAAAGTCACGCTCAATGACCTGACACGCCAGGCGTTGAATGA
GTTTTTGCAACATCACTGCGCAGAACCGCTATCGGTGCTCGGTTGA

Structures

Toxin

• AlphaFold DB: A0A836MG83