Detailed information of TA system TA140757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1893449..1894119
• Replicon: chromosome
• Accession: NZ_CP044345
• Organism: Mycobacterium tuberculosis strain L

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: F6W99_RS09365
• Protein ID: WP_003899954.1
• Coordinates: 1893449..1893793 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: F6W99_RS09370
• Protein ID: WP_003899955.1
• Coordinates: 1893790..1894119 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W99_RS09330	1888522..1889382	+	861	WP_003416628.1	alpha/beta hydrolase	-
F6W99_RS09335	1889477..1889872	+	396	WP_071854247.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
F6W99_RS09345	1890112..1890405	+	294	WP_003416635.1	hypothetical protein	-
F6W99_RS09350	1890693..1891982	+	1290	WP_003416640.1	ATP-binding protein	-
F6W99_RS09355	1892329..1892763	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
F6W99_RS09360	1892766..1893218	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
F6W99_RS09365	1893449..1893793	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F6W99_RS09370	1893790..1894119	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
F6W99_RS09375	1894481..1895742	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-
F6W99_RS09385	1895807..1897068	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-
F6W99_RS09390	1897374..1897721	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
F6W99_RS09395	1897718..1898338	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T140757 WP_003899954.1 NZ_CP044345:1893449-1893793 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T140757 NZ_CP059414:c2487994-2487509 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGGAGCGTTAACCATACGCAAGCGACGGGGAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCTCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT140757 WP_003899955.1 NZ_CP044345:1893790-1894119 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT140757 NZ_CP059414:c2488248-2487982 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6