Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1893449..1894119 Replicon chromosome
Accession NZ_CP044345
Organism Mycobacterium tuberculosis strain L

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag F6W99_RS09365 Protein ID WP_003899954.1
Coordinates 1893449..1893793 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag F6W99_RS09370 Protein ID WP_003899955.1
Coordinates 1893790..1894119 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6W99_RS09330 1888522..1889382 + 861 WP_003416628.1 alpha/beta hydrolase -
F6W99_RS09335 1889477..1889872 + 396 WP_071854247.1 nitroreductase family deazaflavin-dependent oxidoreductase -
F6W99_RS09345 1890112..1890405 + 294 WP_003416635.1 hypothetical protein -
F6W99_RS09350 1890693..1891982 + 1290 WP_003416640.1 ATP-binding protein -
F6W99_RS09355 1892329..1892763 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
F6W99_RS09360 1892766..1893218 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
F6W99_RS09365 1893449..1893793 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F6W99_RS09370 1893790..1894119 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
F6W99_RS09375 1894481..1895742 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
F6W99_RS09385 1895807..1897068 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
F6W99_RS09390 1897374..1897721 + 348 WP_003899956.1 DUF2384 domain-containing protein -
F6W99_RS09395 1897718..1898338 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T140757 WP_003899954.1 NZ_CP044345:1893449-1893793 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T140757 NZ_CP059414:c2487994-2487509 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGGAGCGTTAACCATACGCAAGCGACGGGGAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCTCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT140757 WP_003899955.1 NZ_CP044345:1893790-1894119 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT140757 NZ_CP059414:c2488248-2487982 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References