Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 614041..614960 Replicon chromosome
Accession NZ_CP044345
Organism Mycobacterium tuberculosis strain L

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag F6W99_RS02990 Protein ID WP_003900449.1
Coordinates 614355..614960 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag F6W99_RS02985 Protein ID WP_003410124.1
Coordinates 614041..614346 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6W99_RS02950 609052..610308 - 1257 WP_003410095.1 HNH endonuclease -
F6W99_RS02960 610662..611237 + 576 WP_003410100.1 hypothetical protein -
F6W99_RS02965 611234..612274 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
F6W99_RS02970 612516..613235 + 720 WP_003410108.1 DUF433 domain-containing protein -
F6W99_RS02975 613225..613641 + 417 WP_003410114.1 hypothetical protein -
F6W99_RS02980 613657..613956 - 300 WP_003410120.1 hypothetical protein -
F6W99_RS02985 614041..614346 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
F6W99_RS02990 614355..614960 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F6W99_RS02995 614985..615344 - 360 WP_003410131.1 hypothetical protein -
F6W99_RS03000 615504..616181 - 678 WP_003900450.1 hypothetical protein -
F6W99_RS03005 616244..617505 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
F6W99_RS03010 617545..617727 - 183 WP_151085658.1 hypothetical protein -
F6W99_RS03015 617934..618830 - 897 Protein_576 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T140740 WP_003900449.1 NZ_CP044345:c614960-614355 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T140740 NZ_CP059391:c5213453-5213070 [Klebsiella africana]
ATGACCTGCGGATCTGCGCTTTTTGATACCAACATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCAGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCAAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTAAGCGCTTTTAATATCATCAACATCTCGCAGGATATTGCAGAGCGAAGCGTTGCTCTG
CGGCAGGAGTATAAGCTTAAGCTGCCAGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
AAATACGAGGGATTTTGCCGGCATTCCTGGCGTGGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT140740 WP_003410124.1 NZ_CP044345:c614346-614041 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT140740 NZ_CP059391:c5213695-5213453 [Klebsiella africana]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTTAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCAAGCTAAAGATA
TCATCTCCAGTGCGCTGGGCCTGTGGCAAGACTGCGAAGAAGATGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References