Detailed information of TA system TA140724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4267391..4268118
• Replicon: chromosome
• Accession: NZ_CP044335
• Organism: Enterobacter hormaechei strain AKB48

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D6T76_RS20110
• Protein ID: WP_169724435.1
• Coordinates: 4267807..4268118 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D6T76_RS20105
• Protein ID: WP_169724434.1
• Coordinates: 4267391..4267810 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6T76_RS20095	4265220..4266341	-	1122	WP_169724433.1	glutamate--cysteine ligase	-
D6T76_RS20100	4266447..4267338	+	892	Protein_3917	oxidoreductase	-
D6T76_RS20105	4267391..4267810	-	420	WP_169724434.1	helix-turn-helix domain-containing protein	Antitoxin
D6T76_RS20110	4267807..4268118	-	312	WP_169724435.1	type II toxin-antitoxin system HigB family toxin	Toxin
D6T76_RS20115	4268663..4270153	-	1491	WP_169724436.1	UbiD family decarboxylase	-
D6T76_RS20120	4270164..4270739	-	576	WP_169724437.1	UbiX family flavin prenyltransferase	-
D6T76_RS20125	4270836..4271312	+	477	WP_169724438.1	MarR family transcriptional regulator	-
D6T76_RS20130	4271296..4272312	+	1017	WP_169724439.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-134)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12532.34 Da
• Isoelectric Point: 9.9435

>T140724 WP_169724435.1 NZ_CP044335:c4268118-4267807 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQILDDLYRVLKKEHYPSPDEMRKRFPSPDRMKYREKWWVIDVGGGHLRVMFFADFERGKI
FIKHISTHADYDKLTDYYRRTKE

Nucleotide

• Length: 312 bp

>T140724 NZ_CP059386:c3295429-3295139 [Acinetobacter baumannii]
ATGGTTAAAGTAGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGACATCGTGATGTATCTCAA
TATGGTTCTTGAAGATAATGATCCTGCTGAATTAGCTCATGCCCTTGGTGTCATTGCTAAAGCTAGAGGTATGACCCAAA
TTGCTAAAGAAGCAGGGATCGGACGTGAAGCACTTTATAAAGCACTTCGCCATGATTCAGCACCACGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGCTTAAAACTAAGAGTGCAACACGCCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15363.40 Da
• Isoelectric Point: 4.7132

>AT140724 WP_169724434.1 NZ_CP044335:c4267810-4267391 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFTGSSPSRQDYEDALALVEYLIEHDPDNPLVDMLTAKIDKYENASPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT140724 NZ_CP059386:c3295727-3295431 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTACCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGCATTCGCTTGAA
TCGCCGATTAGATAAGGTTCAACGCGGGAACTGGGGAGACATTAAACCTCTCCAAGATGGTGTTTGGGAAATGAGAGAGT
TCTTCGGTTCAGGATGGAGGATGTATTACATCCAACATGGTGACGTAGTGATTGTAATGCTTGGTGGCGGAGATAAATCA
ACTCAACAACAAGACATCGATCGTGCAGTCAAATTATCGAAAACGTTGGAGGATTAA