Detailed information of TA system TA140720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2904377..2905107
• Replicon: chromosome
• Accession: NZ_CP044335
• Organism: Enterobacter hormaechei strain AKB48

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: D6T76_RS13595
• Protein ID: WP_023302825.1
• Coordinates: 2904377..2904691 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D6T76_RS13600
• Protein ID: WP_022646553.1
• Coordinates: 2904688..2905107 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6T76_RS13570	2900048..2901128	+	1081	Protein_2653	cellulase family glycosylhydrolase	-
D6T76_RS13575	2901034..2902218	-	1185	WP_169723551.1	multidrug efflux MFS transporter EmrD	-
D6T76_RS13580	2902394..2903227	-	834	WP_169723552.1	DMT family transporter	-
D6T76_RS13585	2903290..2903736	-	447	WP_033487022.1	GNAT family N-acetyltransferase	-
D6T76_RS13590	2903801..2903890	-	90	WP_017383683.1	type I toxin-antitoxin system toxin TisB	-
D6T76_RS13595	2904377..2904691	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
D6T76_RS13600	2904688..2905107	+	420	WP_022646553.1	helix-turn-helix domain-containing protein	Antitoxin
D6T76_RS13605	2905254..2905352	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
D6T76_RS13610	2905459..2907147	+	1689	WP_169723553.1	acetolactate synthase large subunit	-
D6T76_RS13615	2907151..2907438	+	288	WP_015570113.1	acetolactate synthase small subunit	-
D6T76_RS13620	2907564..2908157	+	594	WP_060460047.1	transcriptional regulator UhpA	-
D6T76_RS13625	2908154..2909659	+	1506	WP_169723554.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Antitoxin HigA (1-139)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T140720 WP_023302825.1 NZ_CP044335:2904377-2904691 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T140720 NZ_CP059383:c1969268-1969014 [Streptococcus agalactiae]
ATGTTTAATTTTACTGAAGAAGCATGGAAAGATTATGTTTCTTGGCAACAGGAAGATAAAAAAATCTTGAAACGAATTAA
TCGTTTGATAGAAGATATTAAAAGAGACCCATTTGAAGGCATTGGAAAACCAGAACCTTTAAAATACCATTATTCAGGGG
CTTGGTCTCGAAGAATTACAGAAGAACATAGGTTGATTTATATGATAGAAGATGGAGAAATCTATTTTTTATCTTTTAGA
GATCATTATAAGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15480.10 Da
• Isoelectric Point: 5.6761

>AT140720 WP_022646553.1 NZ_CP044335:2904688-2905107 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT140720 NZ_CP059383:c1969527-1969261 [Streptococcus agalactiae]
ATGGAGGCTATTGTTTATTCACATTTTAGAAATAATTTAAAAGATTATATGAAGAAGGTCAATGATGAATTTGAGCCTTT
GATTGTAGTTAATAAAAATCCTGATGAAAATATAGTGGTTTTATCTCAAGATAGCTGGGAAAGTTTACAAGAAACGATTC
GTTTGATGGAAAATGATTATCTATCACATAAGGTTATTAATGGTATATCACAAGTTAAAGAGAAGCAAGTCACTAAACAT
GGTTTAATTGAGGTAGAAGATGTTTAA

Structures

Toxin

• AlphaFold DB: A0A837FIL2