Detailed information of TA system TA140715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1267587..1268247
• Replicon: chromosome
• Accession: NZ_CP044335
• Organism: Enterobacter hormaechei strain AKB48

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J0Q4X0
• Locus tag: D6T76_RS06050
• Protein ID: WP_017383312.1
• Coordinates: 1267894..1268247 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D6T76_RS06045
• Protein ID: WP_169722469.1
• Coordinates: 1267587..1267889 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6T76_RS06030	1263760..1265085	+	1326	WP_169722467.1	SidA/IucD/PvdA family monooxygenase	-
D6T76_RS06035	1265112..1267301	+	2190	WP_169722468.1	TonB-dependent siderophore receptor	-
D6T76_RS06045	1267587..1267889	-	303	WP_169722469.1	XRE family transcriptional regulator	Antitoxin
D6T76_RS06050	1267894..1268247	-	354	WP_017383312.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D6T76_RS06055	1268438..1269388	-	951	WP_169722470.1	HTH-type transcriptional regulator Cbl	-
D6T76_RS06060	1269485..1270402	-	918	WP_169722471.1	nitrogen assimilation transcriptional regulator NAC	-
D6T76_RS06070	1270936..1271868	-	933	WP_169722472.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13537.37 Da
• Isoelectric Point: 9.9538

>T140715 WP_017383312.1 NZ_CP044335:c1268247-1267894 [Enterobacter hormaechei]
VWAIKTTDRFDDWFTSLNDSERASVLAALLVLRERGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREYTRWLNTLKERN

Nucleotide

• Length: 354 bp

>T140715 NZ_CP059359:448620-449009 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11030.82 Da
• Isoelectric Point: 6.4727

>AT140715 WP_169722469.1 NZ_CP044335:c1267889-1267587 [Enterobacter hormaechei]
MGRTTEQLIADEKPEVVASAQAMATDILLNIHLAELREKVQKTQVDMARALGIKQPTVAVMEKAGRDIKLSSLKRYIEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT140715 NZ_CP059359:448356-448613 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: A0A2J0Q4X0