Detailed information of TA system TA140696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 56760..57336
• Replicon: plasmid unnamed1
• Accession: NZ_CP044329
• Organism: Methylocystis rosea strain BRCS1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: F7D13_RS16590
• Protein ID: WP_154454000.1
• Coordinates: 56760..57038 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F7D13_RS16595
• Protein ID: WP_154453910.1
• Coordinates: 57040..57336 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D13_RS16575	53154..53867	-	714	WP_154453907.1	SMC-Scp complex subunit ScpB	-
F7D13_RS16580	53987..55048	-	1062	WP_154453908.1	DUF1403 family protein	-
F7D13_RS16585	55583..56635	+	1053	WP_154453909.1	tyrosine-type recombinase/integrase	-
F7D13_RS16590	56760..57038	+	279	WP_154454000.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F7D13_RS16595	57040..57336	+	297	WP_154453910.1	helix-turn-helix domain-containing protein	Antitoxin
F7D13_RS18370	57511..57849	+	339	WP_195838302.1	DUF433 domain-containing protein	-
F7D13_RS16605	57933..58151	-	219	WP_154453911.1	hypothetical protein	-
F7D13_RS16610	58227..59603	-	1377	WP_154453912.1	replication initiation protein RepC	-
F7D13_RS16615	59756..60751	-	996	WP_154454001.1	plasmid partitioning protein RepB	-
F7D13_RS16620	60830..62041	-	1212	WP_154453913.1	plasmid partitioning protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..195485	195485

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (27-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-75)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10170.80 Da
• Isoelectric Point: 10.0171

>T140696 WP_154454000.1 NZ_CP044329:56760-57038 [Methylocystis rosea]
MTESERSAIVDLLAASPEVGDLIPGTGGLRKVRVPLAGRGKRGGARVIYYVYDQTLPIYLLLAYAKNERDDLSSEQKAVL
RRLVETILARKQ

Nucleotide

• Length: 279 bp

>T140696 NZ_CP059348:c550381-549806 [Providencia rettgeri]
ATGATCCATTCCCCTTTTCCTGTTGTACTAGATGCATGTGTTTTATATCCATCCCTTTTACGGGATTTACTCATGCATCT
AGCCATTACAGGCCTGTATCAAGCAAAATGGACCGATACTATTCAAGACGAATGGCAACGGAATCTACTCAGTAACCGTC
CTGATTTAGCCCAAAAACAGTTAATCAGAACCGCCTCACTAATGAATAATGCGCTACCAGACGCTAGAGTCAGTCAATAT
GAACCATTAATTGAAGGTATTGAATTGCCCGACCTTAATGACCGGCATGTTGTTGCTGCAGCCATAAAATGCCATGCAAA
AATCATTGTGACGCTCAATCTAAAAGATTTTCCAAAAGAATCTATGGATCAATTAGAAATAGAAGCCTTGCATCCAGATG
AGTTTATTTCAGACTTATTTGATTTAAACCATGCTCTGGCTTTATCCGCTGTGCGCCGACAACGACTCAGCTTAACAAAG
CCAGAAATAAATATTAACGAGTACTTTGCCGCCTTGTTACGCCAAGGATTGCCGATGACAGTCAAAGCACTCGAAAAATA
CCAGAGCATGATTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10332.01 Da
• Isoelectric Point: 11.5333

>AT140696 WP_154453910.1 NZ_CP044329:57040-57336 [Methylocystis rosea]
MKEALFNDLVKSLEQAAAHARGQAVPGLQVHPAPTAADIISIRQQAGLTRKQFAAILGTAVGTLRKWEDGSRAPSGAART
LLLVLRRNPKAVLEALAA

Nucleotide

• Length: 297 bp

>AT140696 NZ_CP059348:c550842-550381 [Providencia rettgeri]
ATGACACACTCAATTCTTAATAGTATGAGCCTCCCGGCTCCAAAGGAGATTGAGGCGGCAGTACGCGGACAAAGAGAGCT
TGCAACTTATCTCTCCACTAAGTTAGAAACCCAGCAAATATCCATTAAAGATGCTGATGACAATATTCATCAAATTGAGT
TACCAACATCAGCACTCACATTGTTAATGGCTATTTTGGGTGAGCTAGCAGCAGGTAATGCAGTACAAGTTGTTCCCGTA
CATGCAGAGCTAACAACGCAGGAAGCGGCTAATATTCTTAATGTTTCTCGCCCACATATGGTGAAACTGCTAGAAGAAGG
TAGACTTCCTTTTCATAAAACAGGTCGTCATCGCCGTATTCTTTTTGCAGATTTAATGCAATTCAAAGCTCAACGAGAGA
AAGAGAGCTTACAGGCAATGCAAGCGCTGGTAGACCAAAGTCAGGAGCTTGGTCTTTACTGA