Detailed information of TA system TA140689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 186013..186548
• Replicon: plasmid p15-307-1_01
• Accession: NZ_CP044317
• Organism: Enterococcus faecium strain 15-307-1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: R2PQC0
• Locus tag: CWC51_RS14910
• Protein ID: WP_002314366.1
• Coordinates: 186013..186285 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q3Y220
• Locus tag: CWC51_RS14915
• Protein ID: WP_002297422.1
• Coordinates: 186285..186548 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWC51_RS14870	181119..181568	-	450	WP_010729386.1	TlpA family protein disulfide reductase	-
CWC51_RS14875	181724..182266	-	543	WP_002298088.1	hypothetical protein	-
CWC51_RS14880	182541..183184	+	644	Protein_208	transposase	-
CWC51_RS14885	183260..183940	+	681	WP_002331383.1	IS6-like element IS1216 family transposase	-
CWC51_RS14890	183999..184187	-	189	Protein_210	hypothetical protein	-
CWC51_RS14895	184188..185045	-	858	WP_002292680.1	AAA family ATPase	-
CWC51_RS14900	185331..185617	-	287	Protein_212	type II toxin-antitoxin system RelE/ParE family toxin	-
CWC51_RS14905	185607..185936	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
CWC51_RS14910	186013..186285	-	273	WP_002314366.1	Txe/YoeB family addiction module toxin	Toxin
CWC51_RS14915	186285..186548	-	264	WP_002297422.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CWC51_RS14920	186782..187690	+	909	Protein_216	IS982 family transposase	-
CWC51_RS14925	187693..188868	-	1176	WP_002336205.1	MFS transporter	-
CWC51_RS14930	188871..191120	-	2250	WP_002314387.1	alpha-galactosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..199401	199401
	-	inside	IScluster/Tn	-	-	174450..197149	22699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10780.24 Da
• Isoelectric Point: 9.9724

>T140689 WP_002314366.1 NZ_CP044317:c186285-186013 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T140689 NZ_CP059347:1606106-1606516 [Providencia rettgeri]
GTGGTCCTGTGGAAATCTAATCTCTCCATTTCATGGAAAACTCAATTGTTCTCAACCTGTGTTCATGGGGTTGTTGGGAT
GTTTTTACTATTGGCTCCATGGTCCCCTGATAACTCAATGATATGGTTGCCGCTATTAGTGGTTGTCGTTGCGAGTTGGG
CAAAGAGCCAGAAAAATATCAGTAAAATAAAAGGGGTTGCTGTACTGGTTAATGGCAATAAGGTTCAATGGAAGAAAAAT
GAGTGGAGAATACTGAAAGCGCCTTGGCTTAGCCGTTATGGGGTATTGCTGACACTAGAAGCTTTGCAAGGTAAACCGCA
AAAGGTTCATTTATGGATAGCGAAAGATGCTGTGTCAGAAGAGAATTGGCGTAATTTAAACCAGTTATTACTGCAATACC
CTGATATCTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9959.22 Da
• Isoelectric Point: 4.5370

>AT140689 WP_002297422.1 NZ_CP044317:c186548-186285 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT140689 NZ_CP059347:1605859-1606125 [Providencia rettgeri]
ATGGATATTGAGAATAAAGCGCGAATTCATTGGGCATGCCGTCGTGGTATGCGTGAATTAGATATTTCAATCATGCCGTT
TTTTGAGTATGAATATGATGACCTAACAACTCACGATAAGCAGGTATTTGTCCGTTTATTAGAATGTGCGGACCCTGATT
TATTCAATTGGTTAATGAATCATGGTCGCCCAGATGATGACGAACTTTTTCGTATGATTAAACTGATTCAGGAAAAAAAT
AAAAATCGTGGTCCTGTGGAAATCTAA

Structures

Toxin

• AlphaFold DB: A0A829F704

Antitoxin

• AlphaFold DB: A0A829F006