Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3358886..3359427 Replicon chromosome
Accession NZ_CP044315
Organism Escherichia coli strain SJ7

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag F7D02_RS16960 Protein ID WP_000615976.1
Coordinates 3359149..3359427 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag F7D02_RS16955 Protein ID WP_000729704.1
Coordinates 3358886..3359146 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D02_RS16930 3354669..3355454 - 786 WP_000207567.1 putative lateral flagellar export/assembly protein LafU -
F7D02_RS16935 3355399..3357138 + 1740 WP_001298878.1 flagellar biosynthesis protein FlhA -
F7D02_RS16940 3357243..3357521 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
F7D02_RS16945 3357514..3357870 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
F7D02_RS16950 3357927..3358700 - 774 WP_000543899.1 C40 family peptidase -
F7D02_RS16955 3358886..3359146 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
F7D02_RS16960 3359149..3359427 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
F7D02_RS16965 3359583..3360323 + 741 WP_001225679.1 murein L,D-transpeptidase -
F7D02_RS16970 3360294..3361061 - 768 WP_000333380.1 class II glutamine amidotransferase -
F7D02_RS16975 3361267..3361845 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T140674 WP_000615976.1 NZ_CP044315:3359149-3359427 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T140674 NZ_CP059331:2264899-2265081 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT140674 WP_000729704.1 NZ_CP044315:3358886-3359146 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT140674 NZ_CP059331:2265120-2265539 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References