Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 5019608..5020110 Replicon chromosome
Accession NZ_CP044314
Organism Escherichia coli strain RM9245

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag F7D03_RS25125 Protein ID WP_000767829.1
Coordinates 5019608..5019862 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag F7D03_RS25130 Protein ID WP_001259255.1
Coordinates 5019859..5020110 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D03_RS25095 5014627..5014854 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
F7D03_RS25100 5014868..5015926 - 1059 WP_000492346.1 transport protein YeeE -
F7D03_RS25105 5016101..5017459 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
F7D03_RS25110 5017449..5017511 - 63 WP_010723108.1 membrane protein YoeI -
F7D03_RS25115 5017726..5018655 - 930 WP_000803351.1 LysR family transcriptional regulator -
F7D03_RS25120 5018701..5019525 - 825 WP_064579155.1 SDR family oxidoreductase -
F7D03_RS25125 5019608..5019862 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
F7D03_RS25130 5019859..5020110 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
F7D03_RS25135 5020393..5020443 + 51 WP_001364200.1 his operon leader peptide -
F7D03_RS25140 5020589..5021488 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
F7D03_RS25145 5021494..5022798 + 1305 WP_001369878.1 histidinol dehydrogenase -
F7D03_RS25150 5022795..5023865 + 1071 WP_000108962.1 histidinol-phosphate transaminase -
F7D03_RS25155 5023865..5024932 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T140656 WP_000767829.1 NZ_CP044314:c5019862-5019608 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T140656 NZ_CP059329:c1404826-1404398 [Mannheimia haemolytica]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT140656 WP_001259255.1 NZ_CP044314:c5020110-5019859 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT140656 NZ_CP059329:c1404990-1404826 [Mannheimia haemolytica]
ATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCTTTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGA
TGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAAATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATG
AGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References