Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2974506..2975047 Replicon chromosome
Accession NZ_CP044314
Organism Escherichia coli strain RM9245

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag F7D03_RS14505 Protein ID WP_000615976.1
Coordinates 2974506..2974784 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag F7D03_RS14510 Protein ID WP_000729704.1
Coordinates 2974787..2975047 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D03_RS14490 2972088..2972666 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
F7D03_RS14495 2972872..2973639 + 768 WP_000333380.1 class II glutamine amidotransferase -
F7D03_RS14500 2973610..2974350 - 741 WP_001225679.1 murein L,D-transpeptidase -
F7D03_RS14505 2974506..2974784 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
F7D03_RS14510 2974787..2975047 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
F7D03_RS14515 2975233..2976006 + 774 WP_000543895.1 C40 family peptidase -
F7D03_RS14520 2976063..2976419 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
F7D03_RS14525 2976412..2976690 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
F7D03_RS14530 2976795..2978534 - 1740 WP_001296890.1 flagellar biosynthesis protein FlhA -
F7D03_RS14535 2978479..2979264 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T140643 WP_000615976.1 NZ_CP044314:c2974784-2974506 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T140643 NZ_CP059327:1945226-1945654 [Mannheimia haemolytica]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT140643 WP_000729704.1 NZ_CP044314:c2975047-2974787 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT140643 NZ_CP059327:1945062-1945226 [Mannheimia haemolytica]
ATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCTTTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGA
TGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAAATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATG
AGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References