Detailed information of TA system TA140641

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 2384455..2384952
• Replicon: chromosome
• Accession: NZ_CP044314
• Organism: Escherichia coli strain RM9245

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: -
• Locus tag: F7D03_RS11640
• Protein ID: WP_001173344.1
• Coordinates: 2384779..2384952 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: L4JK92
• Locus tag: F7D03_RS11635
• Protein ID: WP_000398619.1
• Coordinates: 2384455..2384751 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D03_RS11615	2381186..2381905	-	720	WP_000611288.1	two-component system response regulator DcuR	-
F7D03_RS11620	2381902..2383533	-	1632	WP_001216461.1	sensor histidine kinase	-
F7D03_RS11625	2383714..2383944	+	231	WP_000371700.1	(4Fe-4S)-binding protein	-
F7D03_RS11630	2383956..2384228	+	273	WP_000405647.1	N-acetyltransferase	-
F7D03_RS11635	2384455..2384751	+	297	WP_000398619.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
F7D03_RS11640	2384779..2384952	+	174	WP_001173344.1	type V toxin-antitoxin system toxin GhoT	Toxin
F7D03_RS11645	2385071..2386588	-	1518	WP_001295074.1	lysine--tRNA ligase	-
F7D03_RS11650	2386825..2388282	-	1458	WP_000856832.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020087 4238430..4247780 0.007 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021458 4357262..4366612 0.007 View NC_012759 Escherichia coli BW2952 Genomic island TA023874 4289342..4298692 0.007 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285032 4238430..4247780 0.007	inside	Genomic island	-	-	2384455..2393805	9350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6583.12 Da
• Isoelectric Point: 10.2288

>T140641 WP_001173344.1 NZ_CP044314:2384779-2384952 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSVFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T140641 NZ_CP059327:c1666320-1666144 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11467.97 Da
• Isoelectric Point: 4.1705

>AT140641 WP_000398619.1 NZ_CP044314:2384455-2384751 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT140641 NZ_CP059327:c1666088-1665672 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBH6