Detailed information of TA system TA140640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2078016..2078618
• Replicon: chromosome
• Accession: NZ_CP044314
• Organism: Escherichia coli strain RM9245

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: F7D03_RS10210
• Protein ID: WP_000897305.1
• Coordinates: 2078016..2078327 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F7D03_RS10215
• Protein ID: WP_000356397.1
• Coordinates: 2078328..2078618 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D03_RS10185	2073930..2074529	+	600	WP_001295269.1	glucose-1-phosphatase	-
F7D03_RS10190	2074523..2075395	+	873	WP_000920762.1	virulence factor BrkB family protein	-
F7D03_RS10195	2075392..2075829	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
F7D03_RS10200	2075874..2076815	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
F7D03_RS10205	2076879..2077787	-	909	WP_001162704.1	alpha/beta hydrolase	-
F7D03_RS10210	2078016..2078327	+	312	WP_000897305.1	hypothetical protein	Toxin
F7D03_RS10215	2078328..2078618	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
F7D03_RS10220	2079223..2079441	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
F7D03_RS10225	2079660..2079902	+	243	WP_001087409.1	hypothetical protein	-
F7D03_RS10230	2080232..2081161	-	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
F7D03_RS10235	2081158..2081793	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
F7D03_RS10240	2081790..2082692	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T140640 WP_000897305.1 NZ_CP044314:2078016-2078327 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T140640 NZ_CP059327:c1520130-1519861 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GTTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT140640 WP_000356397.1 NZ_CP044314:2078328-2078618 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT140640 NZ_CP059327:c1520383-1520117 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7