Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1269007..1269806 Replicon chromosome
Accession NZ_CP044314
Organism Escherichia coli strain RM9245

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag F7D03_RS06215 Protein ID WP_000347266.1
Coordinates 1269342..1269806 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag F7D03_RS06210 Protein ID WP_001307405.1
Coordinates 1269007..1269342 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D03_RS06195 1264792..1265562 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
F7D03_RS06200 1265578..1266912 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
F7D03_RS06205 1267287..1268858 + 1572 WP_001273741.1 galactarate dehydratase -
F7D03_RS06210 1269007..1269342 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
F7D03_RS06215 1269342..1269806 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
F7D03_RS06220 1269861..1270670 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
F7D03_RS06225 1270919..1272199 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
F7D03_RS06230 1272222..1272695 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
F7D03_RS06235 1272706..1273485 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
F7D03_RS06240 1273475..1274353 + 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
F7D03_RS06245 1274371..1274805 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1258245..1269806 11561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T140638 WP_000347266.1 NZ_CP044314:1269342-1269806 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T140638 NZ_CP059327:177611-177907 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT140638 WP_001307405.1 NZ_CP044314:1269007-1269342 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT140638 NZ_CP059327:177891-178133 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGAGTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References