Detailed information of TA system TA140628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3511750..3512352
• Replicon: chromosome
• Accession: NZ_CP044313
• Organism: Escherichia coli strain RM11911

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: F7D04_RS17490
• Protein ID: WP_000897305.1
• Coordinates: 3511750..3512061 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F7D04_RS17495
• Protein ID: WP_000356397.1
• Coordinates: 3512062..3512352 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D04_RS17460	3506780..3507565	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
F7D04_RS17465	3507664..3508263	+	600	WP_001315111.1	glucose-1-phosphatase	-
F7D04_RS17470	3508257..3509129	+	873	WP_000920762.1	virulence factor BrkB family protein	-
F7D04_RS17475	3509126..3509563	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
F7D04_RS17480	3509608..3510549	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
F7D04_RS17485	3510613..3511521	-	909	WP_001162704.1	alpha/beta hydrolase	-
F7D04_RS17490	3511750..3512061	+	312	WP_000897305.1	hypothetical protein	Toxin
F7D04_RS17495	3512062..3512352	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
F7D04_RS17500	3512957..3513175	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
F7D04_RS17505	3513394..3513636	+	243	WP_001086388.1	hypothetical protein	-
F7D04_RS17510	3513865..3514845	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
F7D04_RS17515	3515292..3516221	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
F7D04_RS17520	3516218..3516853	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3513865..3514845	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T140628 WP_000897305.1 NZ_CP044313:3511750-3512061 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T140628 NZ_CP059325:2727242-2727511 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT140628 WP_000356397.1 NZ_CP044313:3512062-3512352 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT140628 NZ_CP059325:2726972-2727238 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7