Detailed information of TA system TA140622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2092572..2093299
• Replicon: chromosome
• Accession: NZ_CP044313
• Organism: Escherichia coli strain RM11911

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: F7D04_RS10535
• Protein ID: WP_000547564.1
• Coordinates: 2092988..2093299 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F7D04_RS10530
• Protein ID: WP_000126294.1
• Coordinates: 2092572..2092991 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D04_RS10510	2087622..2088149	-	528	WP_001078777.1	electron transport protein HydN	-
F7D04_RS10515	2088298..2089308	-	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
F7D04_RS10520	2089568..2091025	+	1458	WP_050939299.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
F7D04_RS10525	2091034..2092458	+	1425	WP_000110320.1	6-phospho-beta-glucosidase AscB	-
F7D04_RS10530	2092572..2092991	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
F7D04_RS10535	2092988..2093299	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
F7D04_RS10540	2093473..2094234	-	762	WP_001026446.1	hypothetical protein	-
F7D04_RS10545	2094259..2094729	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
F7D04_RS10550	2094722..2095132	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
F7D04_RS10555	2095129..2095896	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
F7D04_RS10560	2095896..2096438	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
F7D04_RS10565	2096448..2098157	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T140622 WP_000547564.1 NZ_CP044313:c2093299-2092988 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T140622 NZ_CP059325:794025-794453 [Mannheimia haemolytica]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT140622 WP_000126294.1 NZ_CP044313:c2092991-2092572 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT140622 NZ_CP059325:793861-794025 [Mannheimia haemolytica]
ATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCTTTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGA
TGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAAATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATG
AGTAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5