Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1287281..1287783 Replicon chromosome
Accession NZ_CP044313
Organism Escherichia coli strain RM11911

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag F7D04_RS06865 Protein ID WP_000767829.1
Coordinates 1287281..1287535 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag F7D04_RS06870 Protein ID WP_001259255.1
Coordinates 1287532..1287783 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D04_RS06835 1282296..1282523 - 228 WP_000234896.1 sulfurtransferase TusA -
F7D04_RS06840 1282537..1283595 - 1059 WP_000492339.1 transport protein YeeE -
F7D04_RS06845 1283774..1285132 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
F7D04_RS06850 1285122..1285184 - 63 WP_010723108.1 membrane protein YoeI -
F7D04_RS06855 1285399..1286328 - 930 WP_000803351.1 LysR family transcriptional regulator -
F7D04_RS06860 1286374..1287198 - 825 WP_000754737.1 SDR family oxidoreductase -
F7D04_RS06865 1287281..1287535 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
F7D04_RS06870 1287532..1287783 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
F7D04_RS06875 1288066..1288116 + 51 WP_001364200.1 his operon leader peptide -
F7D04_RS06880 1288262..1289161 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
F7D04_RS06885 1289167..1290465 + 1299 WP_000009595.1 histidinol dehydrogenase -
F7D04_RS06890 1290468..1291538 + 1071 WP_001468894.1 histidinol-phosphate transaminase -
F7D04_RS06895 1291538..1292605 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T140620 WP_000767829.1 NZ_CP044313:c1287535-1287281 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T140620 NZ_CP059325:c522687-522418 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAATAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GCTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT140620 WP_001259255.1 NZ_CP044313:c1287783-1287532 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT140620 NZ_CP059325:c522940-522674 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References