Detailed information of TA system TA140606

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 480365..480736
• Replicon: chromosome
• Accession: NZ_CP044313
• Organism: Escherichia coli strain RM11911

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: F7D04_RS02580
• Protein ID: WP_021500490.1
• Coordinates: 480542..480736 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	480365..480543 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D04_RS02555	476320..477693	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
F7D04_RS02560	477822..478757	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
F7D04_RS02565	478809..480044	-	1236	WP_072017917.1	site-specific integrase	-
F7D04_RS02570	480046..480261	-	216	WP_000079604.1	excisionase XisR	-
F7D04_RS02575	480361..480549	-	189	WP_151039308.1	DUF1187 family protein	-
-	480365..480543	+	179	NuclAT_0	-	Antitoxin
-	480365..480543	+	179	NuclAT_0	-	Antitoxin
-	480365..480543	+	179	NuclAT_0	-	Antitoxin
-	480365..480543	+	179	NuclAT_0	-	Antitoxin
F7D04_RS02580	480542..480736	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
F7D04_RS02585	480800..481888	-	1089	WP_021529594.1	recombinase RecT	-
F7D04_RS02590	481903..484977	-	3075	WP_042973008.1	exodeoxyribonuclease VIII	-
F7D04_RS02595	485079..485353	-	275	Protein_510	hypothetical protein	-
F7D04_RS02600	485428..485598	-	171	WP_001349883.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	478809..525221	46412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T140606 WP_021500490.1 NZ_CP044313:c480736-480542 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T140606 NZ_CP059323:c219285-219103 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT140606 NZ_CP044313:480365-480543 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTTGCATTCCGATTTGGTTAACTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin