Detailed information of TA system TA140604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5095584..5096186
• Replicon: chromosome
• Accession: NZ_CP044312
• Organism: Escherichia coli strain RM13745

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: F7D05_RS25000
• Protein ID: WP_000897305.1
• Coordinates: 5095584..5095895 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F7D05_RS25005
• Protein ID: WP_000356397.1
• Coordinates: 5095896..5096186 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D05_RS24970	5090614..5091399	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
F7D05_RS24975	5091498..5092097	+	600	WP_001315111.1	glucose-1-phosphatase	-
F7D05_RS24980	5092091..5092963	+	873	WP_000920762.1	virulence factor BrkB family protein	-
F7D05_RS24985	5092960..5093397	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
F7D05_RS24990	5093442..5094383	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
F7D05_RS24995	5094447..5095355	-	909	WP_001162704.1	alpha/beta hydrolase	-
F7D05_RS25000	5095584..5095895	+	312	WP_000897305.1	hypothetical protein	Toxin
F7D05_RS25005	5095896..5096186	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
F7D05_RS25010	5096791..5097009	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
F7D05_RS25015	5097228..5097470	+	243	WP_001086388.1	hypothetical protein	-
F7D05_RS25020	5097699..5098679	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
F7D05_RS25025	5099126..5100055	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
F7D05_RS25030	5100052..5100687	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5097699..5098679	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T140604 WP_000897305.1 NZ_CP044312:5095584-5095895 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T140604 NZ_CP059319:5718830-5719114 [Rhizorhabdus wittichii]
GTGGCAAGCCCGACGCTTGAATGGAGCGAGGCTGCCCGCAAGGACCTGCTGTCGATTGTCGATTTCATCGCCGACGATAA
TCCCGATGCGGCGCTGAAGCTGCTCGACGACATAGAAAACAAGGCGGAGCAATTACTCCACCATCCCCGACTATACGAGG
TGGGGCGTGTCGATGGCACACGGGAAATGCTCGTCCGGCAAAATTACATCGTCGTTTATGCGGAAGACGAACAGCGTGTC
CTCATCCTTCGCGTGTTGCACGGCGCGCAGTTCTGGCCGCGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT140604 WP_000356397.1 NZ_CP044312:5095896-5096186 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT140604 NZ_CP059319:5718544-5718849 [Rhizorhabdus wittichii]
ATGGCTGGCCGCACGTCCATGCTGCATGTCAGGGTCGATGAGGAACTGAAAGCGCGCGCATCCCAAACGCTCGCCGATGT
TGGGCTGAGCGTGTCCGATGCGGTGAGGATATTGCTCGCACGGGTCGCCAAGGAGGGCGGGCTGCCGGCCGGCCTGACCG
TCGATCCCGAGACGCATGATGCCTGGTTCCGGGCAAAGGTGCGGGAGGCGCTGGACGACGGCGGCCCAACCCGTCCGCAA
GCGAGGGTCATGGACGAAGCGCGCGCGCTCGTGGTGGGAAAGCGGCGTGGCAAGCCCGACGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7