Detailed information of TA system TA140598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3676186..3676913
• Replicon: chromosome
• Accession: NZ_CP044312
• Organism: Escherichia coli strain RM13745

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: F7D05_RS18040
• Protein ID: WP_000547564.1
• Coordinates: 3676602..3676913 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F7D05_RS18035
• Protein ID: WP_000126294.1
• Coordinates: 3676186..3676605 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D05_RS18015	3671236..3671763	-	528	WP_001078777.1	electron transport protein HydN	-
F7D05_RS18020	3671912..3672922	-	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
F7D05_RS18025	3673182..3674639	+	1458	WP_050939299.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
F7D05_RS18030	3674648..3676072	+	1425	WP_000110320.1	6-phospho-beta-glucosidase AscB	-
F7D05_RS18035	3676186..3676605	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
F7D05_RS18040	3676602..3676913	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
F7D05_RS18045	3677087..3677848	-	762	WP_001026446.1	hypothetical protein	-
F7D05_RS18050	3677873..3678343	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
F7D05_RS18055	3678336..3678746	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
F7D05_RS18060	3678743..3679510	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
F7D05_RS18065	3679510..3680052	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
F7D05_RS18070	3680062..3681771	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T140598 WP_000547564.1 NZ_CP044312:c3676913-3676602 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T140598 NZ_CP059318:489473-489823 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT140598 WP_000126294.1 NZ_CP044312:c3676605-3676186 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT140598 NZ_CP059318:489186-489467 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5