Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2870876..2871378 Replicon chromosome
Accession NZ_CP044312
Organism Escherichia coli strain RM13745

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag F7D05_RS14380 Protein ID WP_000767829.1
Coordinates 2870876..2871130 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag F7D05_RS14385 Protein ID WP_001259255.1
Coordinates 2871127..2871378 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D05_RS14350 2865891..2866118 - 228 WP_000234896.1 sulfurtransferase TusA -
F7D05_RS14355 2866132..2867190 - 1059 WP_000492339.1 transport protein YeeE -
F7D05_RS14360 2867369..2868727 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
F7D05_RS14365 2868717..2868779 - 63 WP_010723108.1 membrane protein YoeI -
F7D05_RS14370 2868994..2869923 - 930 WP_000803351.1 LysR family transcriptional regulator -
F7D05_RS14375 2869969..2870793 - 825 WP_000754737.1 SDR family oxidoreductase -
F7D05_RS14380 2870876..2871130 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
F7D05_RS14385 2871127..2871378 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
F7D05_RS14390 2871661..2871711 + 51 WP_001364200.1 his operon leader peptide -
F7D05_RS14395 2871857..2872756 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
F7D05_RS14400 2872762..2874060 + 1299 WP_000009595.1 histidinol dehydrogenase -
F7D05_RS14405 2874063..2875133 + 1071 WP_001468894.1 histidinol-phosphate transaminase -
F7D05_RS14410 2875133..2876200 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T140596 WP_000767829.1 NZ_CP044312:c2871130-2870876 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T140596 NZ_CP059317:c4934007-4933132 [Klebsiella pneumoniae]
ATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATTTGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGA
TTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGGTGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGG
ATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAGGCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAG
ATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAAGATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACC
ACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTTTTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCT
ACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCATCCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAG
GAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTATTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCC
TCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAATTCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGA
CAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCCTGTATCACACAACAGGATGTCGATTTTTTACTGTCATTTAAA
CGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCGTATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATAT
TCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAGATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT140596 WP_001259255.1 NZ_CP044312:c2871378-2871127 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT140596 NZ_CP059317:c4934813-4934055 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References