Detailed information of TA system TA140582

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2065244..2065615
• Replicon: chromosome
• Accession: NZ_CP044312
• Organism: Escherichia coli strain RM13745

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: F7D05_RS10100
• Protein ID: WP_021500490.1
• Coordinates: 2065421..2065615 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2065244..2065422 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D05_RS10075	2061199..2062572	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
F7D05_RS10080	2062701..2063636	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
F7D05_RS10085	2063688..2064923	-	1236	WP_072017917.1	site-specific integrase	-
F7D05_RS10090	2064925..2065140	-	216	WP_000079604.1	excisionase XisR	-
F7D05_RS10095	2065240..2065428	-	189	WP_151039308.1	DUF1187 family protein	-
-	2065244..2065422	+	179	NuclAT_0	-	Antitoxin
-	2065244..2065422	+	179	NuclAT_0	-	Antitoxin
-	2065244..2065422	+	179	NuclAT_0	-	Antitoxin
-	2065244..2065422	+	179	NuclAT_0	-	Antitoxin
F7D05_RS10100	2065421..2065615	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
F7D05_RS10105	2065679..2066767	-	1089	WP_021529594.1	recombinase RecT	-
F7D05_RS10110	2066782..2069856	-	3075	WP_042973008.1	exodeoxyribonuclease VIII	-
F7D05_RS10115	2069958..2070232	-	275	Protein_1975	hypothetical protein	-
F7D05_RS10120	2070307..2070477	-	171	WP_001349883.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2063688..2110099	46411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T140582 WP_021500490.1 NZ_CP044312:c2065615-2065421 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T140582 NZ_CP059312:c4889500-4888625 [Klebsiella pneumoniae]
ATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATTTGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGA
TTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGGTGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGG
ATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAGGCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAG
ATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAAGATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACC
ACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTTTTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCT
ACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCATCCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAG
GAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTATTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCC
TCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAATTCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGA
CAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCCTGTATCACACAACAGGATGTCGATTTTTTACTGTCATTTAAA
CGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCGTATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATAT
TCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAGATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT140582 NZ_CP044312:2065244-2065422 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTTGCATTCCGATTTGGTTAACTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin