Detailed information of TA system TA140573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4343210..4343812
• Replicon: chromosome
• Accession: NZ_CP044311
• Organism: Escherichia coli strain RM13752

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: F7D06_RS21390
• Protein ID: WP_000897305.1
• Coordinates: 4343210..4343521 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F7D06_RS21395
• Protein ID: WP_000356397.1
• Coordinates: 4343522..4343812 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D06_RS21360	4338240..4339025	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
F7D06_RS21365	4339124..4339723	+	600	WP_001315111.1	glucose-1-phosphatase	-
F7D06_RS21370	4339717..4340589	+	873	WP_000920762.1	virulence factor BrkB family protein	-
F7D06_RS21375	4340586..4341023	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
F7D06_RS21380	4341068..4342009	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
F7D06_RS21385	4342073..4342981	-	909	WP_001162704.1	alpha/beta hydrolase	-
F7D06_RS21390	4343210..4343521	+	312	WP_000897305.1	hypothetical protein	Toxin
F7D06_RS21395	4343522..4343812	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
F7D06_RS21400	4344417..4344635	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
F7D06_RS21405	4344854..4345096	+	243	WP_001086388.1	hypothetical protein	-
F7D06_RS21410	4345325..4346305	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
F7D06_RS21415	4346752..4347681	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
F7D06_RS21420	4347678..4348313	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4345325..4346305	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T140573 WP_000897305.1 NZ_CP044311:4343210-4343521 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T140573 NZ_CP059312:358430-358777 [Klebsiella pneumoniae]
ATGTGGGATGTGGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATAAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT140573 WP_000356397.1 NZ_CP044311:4343522-4343812 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT140573 NZ_CP059312:358777-359076 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7