Detailed information of TA system TA140567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2731290..2732017
• Replicon: chromosome
• Accession: NZ_CP044311
• Organism: Escherichia coli strain RM13752

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: F7D06_RS13535
• Protein ID: WP_000547564.1
• Coordinates: 2731290..2731601 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F7D06_RS13540
• Protein ID: WP_000126294.1
• Coordinates: 2731598..2732017 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D06_RS13505	2726432..2728141	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
F7D06_RS13510	2728151..2728693	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
F7D06_RS13515	2728693..2729460	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
F7D06_RS13520	2729457..2729867	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
F7D06_RS13525	2729860..2730330	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
F7D06_RS13530	2730355..2731116	+	762	WP_001026446.1	hypothetical protein	-
F7D06_RS13535	2731290..2731601	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
F7D06_RS13540	2731598..2732017	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
F7D06_RS13545	2732131..2733555	-	1425	WP_000110320.1	6-phospho-beta-glucosidase AscB	-
F7D06_RS13550	2733564..2735021	-	1458	WP_050939299.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
F7D06_RS13555	2735281..2736291	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
F7D06_RS13560	2736440..2736967	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T140567 WP_000547564.1 NZ_CP044311:2731290-2731601 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T140567 NZ_CP059303:c5604-5209 [Acinetobacter baumannii]
ATGATTTACCTCTTAGACACAAATATTTGTATCTATGTCATCAACAATAAGCCTCAACACGTTTTTGAACGCTTTAAGCA
ATATCAACTCGGCCAGCTTGCGATTTCAAGTATCACGGCATCTGAGTTGGCTTTCGGTGTAGAGAAGTCTGGATCTGAAC
GCAATAAACAGGCGTTAAACAAGTTTTTGTCACCTTTAGAGATTCTTCCTTACGATGAACAGGCCATTTGGCATTACGCC
AAATTACGCCAGAATCTTCAATCTACAGGTAAACCGATTGGCAGCTTAGATATGCTGATTGCTGCTCATGCCTTGGCATT
AGATGTTGTATTAGTTACGAACAATACAAAAGAATTTGAACGGATTGATGGTCTGAAGCTAGAGAATTGGGTCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT140567 WP_000126294.1 NZ_CP044311:2731598-2732017 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT140567 NZ_CP059303:c5831-5601 [Acinetobacter baumannii]
ATGGAAATTGCGAAAGTATTTCAAACTGGTCGCAGTCAGGCTGTTCGCTTGCCTAAAGCCTTTCGCTTTAATGGCACGGA
AGTGGCCATCAAAAGCTTCGGACGTGGCGTTTTGTTGATGCCAATCGATAATCCTTGGGATGTAATGCTTGAAGCAGTGA
ATGAGTTCGAATCTGGATTCCAGTTAGAACGTGCTGATCAGGGGGAACAGTTCCGTGAGGAGTTCAAATGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5