Detailed information of TA system TA140543

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4384128..4384499
• Replicon: chromosome
• Accession: NZ_CP044293
• Organism: Escherichia coli strain P276M

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: F6P95_RS21215
• Protein ID: WP_001317028.1
• Coordinates: 4384305..4384499 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4384128..4384306 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6P95_RS21190	4380083..4381456	+	1374	WP_000123779.1	ATP-dependent RNA helicase DbpA	-
F6P95_RS21195	4381585..4382520	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
F6P95_RS21200	4382572..4383807	-	1236	WP_000040852.1	site-specific integrase	-
F6P95_RS21205	4383809..4384024	-	216	WP_000079604.1	excisionase XisR	-
F6P95_RS21210	4384103..4384312	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	4384128..4384306	+	179	NuclAT_0	-	Antitoxin
-	4384128..4384306	+	179	NuclAT_0	-	Antitoxin
-	4384128..4384306	+	179	NuclAT_0	-	Antitoxin
-	4384128..4384306	+	179	NuclAT_0	-	Antitoxin
F6P95_RS21215	4384305..4384499	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
F6P95_RS21220	4384556..4385365	-	810	WP_157716978.1	recombination protein RecT	-
F6P95_RS21225	4385358..4387958	-	2601	WP_024232464.1	exodeoxyribonuclease VIII	-
F6P95_RS21230	4388060..4388335	-	276	WP_000632297.1	protein RacC	-
F6P95_RS21235	4388410..4388580	-	171	WP_001352098.1	conserved protein, Rac prophage	-
F6P95_RS21240	4388580..4388801	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4375313..4407414	32101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T140543 WP_001317028.1 NZ_CP044293:c4384499-4384305 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T140543 NZ_CP059297:c309700-309335 [Brevundimonas sp. AJA228-03]
ATGGTGAAGGCGCTGTTCGACACCAATATTCTGATCGATCATCTGAACGCCGCGCCGGAGGCCGATGCCGAGATCGATCG
GTACGACGACCGCGCCATAAGCATCATTTCCTGGATCGAGGTGATGGCGGGGGCCGGCCCCGATCTGGCCGAGCCGACGC
GGACCTATCTTGACGGCTATCAGATCGTCGGTCTCGAAGACCGGATCGCCGAACAGGCCGCGTCGCTACGCCAGTCTCGC
CGTCTGAAACTGCCGGACGCCGTCATCTGGGCGACCGCCCAGACGACGGGTCGTCTGCTGGTCACCCGAAACACGAAGGA
TTTCCCGGCCGACGATCCGGGGGTTCGGGCACCCTACAGGATTTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT140543 NZ_CP044293:4384128-4384306 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin