Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2725863..2726360 Replicon chromosome
Accession NZ_CP044293
Organism Escherichia coli strain P276M

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag F6P95_RS13175 Protein ID WP_001173343.1
Coordinates 2726187..2726360 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2U9LI53
Locus tag F6P95_RS13170 Protein ID WP_071597404.1
Coordinates 2725863..2726159 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6P95_RS13150 2722594..2723313 - 720 WP_000611288.1 two-component system response regulator DcuR -
F6P95_RS13155 2723310..2724941 - 1632 WP_001565376.1 sensor histidine kinase -
F6P95_RS13160 2725122..2725352 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
F6P95_RS13165 2725364..2725636 + 273 WP_000405647.1 N-acetyltransferase -
F6P95_RS13170 2725863..2726159 + 297 WP_071597404.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
F6P95_RS13175 2726187..2726360 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
F6P95_RS13180 2726484..2728001 - 1518 WP_001295074.1 lysine--tRNA ligase -
F6P95_RS13185 2728238..2729695 - 1458 WP_072778207.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2725863..2735218 9355


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(6-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T140536 WP_001173343.1 NZ_CP044293:2726187-2726360 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T140536 NZ_CP059295:3363029-3363131 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11495.99 Da        Isoelectric Point: 4.1705

>AT140536 WP_071597404.1 NZ_CP044293:2725863-2726159 [Escherichia coli]
MEGKNRFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT140536 NZ_CP059295:c3363138-3362993 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LI53

References