140511

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 181278..181813 Replicon plasmid pHVH-V1836-1
Accession NZ_CP044265
Organism Enterococcus faecium strain V1836

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag F6439_RS15370 Protein ID WP_002314366.1
Coordinates 181278..181550 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag F6439_RS15375 Protein ID WP_002297422.1
Coordinates 181550..181813 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6439_RS15335 176834..177477 + 644 Protein_180 transposase -
F6439_RS15340 177553..178233 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
F6439_RS15345 178288..178644 - 357 Protein_182 hypothetical protein -
F6439_RS15350 178904..179452 - 549 WP_002292681.1 hypothetical protein -
F6439_RS15355 179453..180310 - 858 WP_002292680.1 AAA family ATPase -
F6439_RS15360 180596..180882 - 287 Protein_185 type II toxin-antitoxin system RelE/ParE family toxin -
F6439_RS15365 180872..181201 - 330 WP_002292678.1 AbrB family transcriptional regulator -
F6439_RS15370 181278..181550 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
F6439_RS15375 181550..181813 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
F6439_RS15380 182047..182955 + 909 Protein_189 IS982 family transposase -
F6439_RS15385 182958..184133 - 1176 WP_002336205.1 MFS transporter -
F6439_RS15390 184136..186385 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..194666 194666
- inside IScluster/Tn - - 167862..192414 24552


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T140511 WP_002314366.1 NZ_CP044265:c181550-181278 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T140511 NZ_CP059288:729728-730024 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT140511 WP_002297422.1 NZ_CP044265:c181813-181550 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT140511 NZ_CP059288:730026-730421 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References