Detailed information of TA system TA140505

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 143326..143579
• Replicon: plasmid pKPC-2-KP65
• Accession: NZ_CP044259
• Organism: Klebsiella pneumoniae strain KP65

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: F6W81_RS28270
• Protein ID: WP_001312851.1
• Coordinates: 143430..143579 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	143326..143385 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W81_RS28225	138986..139051	-	66	Protein_188	helix-turn-helix domain-containing protein	-
F6W81_RS28230	139104..139808	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
F6W81_RS28235	139872..140033	+	162	Protein_190	DNA helicase	-
F6W81_RS28240	140053..140799	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
F6W81_RS28245	140854..141414	+	561	WP_000139328.1	fertility inhibition protein FinO	-
F6W81_RS28250	141546..141746	+	201	WP_015059022.1	hypothetical protein	-
F6W81_RS28255	142132..142731	+	600	WP_032083981.1	hypothetical protein	-
F6W81_RS28260	142895..143125	+	231	WP_001736714.1	hypothetical protein	-
-	143326..143385	-	60	NuclAT_1	-	Antitoxin
-	143326..143385	-	60	NuclAT_1	-	Antitoxin
-	143326..143385	-	60	NuclAT_1	-	Antitoxin
-	143326..143385	-	60	NuclAT_1	-	Antitoxin
F6W81_RS28270	143430..143579	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
F6W81_RS28275	143863..144111	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2	-	1..144422	144422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T140505 WP_001312851.1 NZ_CP044259:143430-143579 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T140505 NZ_CP059285:c73889-73731 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT140505 NZ_CP044259:c143385-143326 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin