Detailed information of TA system TA140501

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 80474..80743
• Replicon: plasmid pKPC-2-KP65
• Accession: NZ_CP044259
• Organism: Klebsiella pneumoniae strain KP65

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: F6W81_RS27800
• Protein ID: WP_001312861.1
• Coordinates: 80585..80743 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	80474..80539 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W81_RS27775	76184..76711	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
F6W81_RS27780	76769..77002	+	234	WP_000006003.1	DUF905 family protein	-
F6W81_RS27785	77063..79086	+	2024	Protein_106	ParB/RepB/Spo0J family partition protein	-
F6W81_RS27790	79155..79589	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
F6W81_RS27795	79586..80305	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	80317..80541	+	225	NuclAT_0	-	-
-	80317..80541	+	225	NuclAT_0	-	-
-	80317..80541	+	225	NuclAT_0	-	-
-	80317..80541	+	225	NuclAT_0	-	-
-	80474..80539	+	66	-	-	Antitoxin
F6W81_RS27800	80585..80743	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
F6W81_RS28945	80981..81358	-	378	Protein_110	hypothetical protein	-
F6W81_RS27820	81658..81954	+	297	WP_001272251.1	hypothetical protein	-
F6W81_RS27825	82065..82886	+	822	WP_001234445.1	DUF945 domain-containing protein	-
F6W81_RS27830	83183..83830	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
F6W81_RS27835	84107..84490	+	384	WP_000124981.1	relaxosome protein TraM	-
F6W81_RS27840	84681..85367	+	687	WP_015059009.1	PAS domain-containing protein	-
F6W81_RS27845	85461..85688	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2	-	1..144422	144422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T140501 WP_001312861.1 NZ_CP044259:80585-80743 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T140501 NZ_CP059283:c3665360-3664962 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT140501 NZ_CP044259:80474-80539 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin