Detailed information of TA system TA140479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 611799..612315
• Replicon: chromosome
• Accession: NZ_CP044236
• Organism: Lacticaseibacillus paracasei strain WX322

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A243Q0H7
• Locus tag: F0M22_RS02970
• Protein ID: WP_003573774.1
• Coordinates: 611799..612080 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: F0M22_RS02975
• Protein ID: WP_016363783.1
• Coordinates: 612073..612315 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0M22_RS02960	609377..610072	+	696	WP_242755738.1	hypothetical protein	-
F0M22_RS02965	610069..610839	-	771	WP_242755740.1	hypothetical protein	-
F0M22_RS02970	611799..612080	-	282	WP_003573774.1	type II toxin-antitoxin system YafQ family toxin	Toxin
F0M22_RS02975	612073..612315	-	243	WP_016363783.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
F0M22_RS02980	612442..612810	-	369	WP_242755742.1	YxeA family protein	-
F0M22_RS02985	613015..613383	-	369	WP_242755742.1	YxeA family protein	-
F0M22_RS02990	613678..614028	-	351	WP_016387184.1	hypothetical protein	-
F0M22_RS02995	614276..615520	+	1245	WP_242755749.1	ISL3 family transposase	-
F0M22_RS03000	615662..615751	+	90	Protein_597	IS30 family transposase	-
F0M22_RS03005	615793..616260	-	468	WP_003582565.1	DNA starvation/stationary phase protection protein	-
F0M22_RS03010	616503..616754	+	252	WP_002816285.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	539207..648356	109149
	-	flank	IS/Tn	-	-	616503..616754	251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11371.98 Da
• Isoelectric Point: 9.5081

>T140479 WP_003573774.1 NZ_CP044236:c612080-611799 [Lacticaseibacillus paracasei]
MNKFVYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T140479 NZ_CP059281:c3450367-3450265 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9217.44 Da
• Isoelectric Point: 5.1548

>AT140479 WP_016363783.1 NZ_CP044236:c612315-612073 [Lacticaseibacillus paracasei]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT140479 NZ_CP059281:3450227-3450495 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A243Q0H7