Detailed information of TA system TA140476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 569836..570348
• Replicon: chromosome
• Accession: NZ_CP044230
• Organism: Streptococcus sp. LPB0220

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: I1ZKJ4
• Locus tag: LPB220_RS03065
• Protein ID: WP_013903192.1
• Coordinates: 570094..570348 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LPB220_RS03060
• Protein ID: WP_191904618.1
• Coordinates: 569836..570090 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPB220_RS03025	565129..565440	+	312	WP_150905466.1	ribosome assembly RNA-binding protein YhbY	-
LPB220_RS03030	565462..566094	+	633	WP_003012335.1	nicotinate-nucleotide adenylyltransferase	-
LPB220_RS03035	566091..566687	+	597	WP_150905468.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LPB220_RS03040	566688..567188	+	501	WP_150905470.1	cysteine hydrolase	-
LPB220_RS03045	567203..567556	+	354	WP_150905472.1	ribosome silencing factor	-
LPB220_RS03050	567765..568505	+	741	WP_150905474.1	class I SAM-dependent methyltransferase	-
LPB220_RS03055	568512..569606	+	1095	WP_150905476.1	nucleotidyltransferase	-
LPB220_RS03060	569836..570090	+	255	WP_191904618.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LPB220_RS03065	570094..570348	+	255	WP_013903192.1	Txe/YoeB family addiction module toxin	Toxin
LPB220_RS03070	570523..571239	+	717	WP_049514178.1	YebC/PmpR family DNA-binding transcriptional regulator	-
LPB220_RS03075	571292..571618	-	327	WP_003005875.1	hypothetical protein	-
LPB220_RS03080	571599..571970	-	372	WP_013903193.1	hypothetical protein	-
LPB220_RS03085	571967..573379	-	1413	WP_150905480.1	Y-family DNA polymerase	-
LPB220_RS03090	573382..574059	-	678	WP_041826243.1	XRE family transcriptional regulator	-
LPB220_RS03095	574219..575211	+	993	WP_003005888.1	aspartate--ammonia ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10082.55 Da
• Isoelectric Point: 7.9789

>T140476 WP_013903192.1 NZ_CP044230:570094-570348 [Streptococcus sp. LPB0220]
MLLKFTEDAWKDYCYWQSQDKKTLKRINTLIKEIQRDPFAGIGKPEPLKYDFQGAWSRRIDAENRLIYMLDESGVAFLSF
KDHY

Nucleotide

• Length: 255 bp

>T140476 NZ_CP059281:c2726685-2726407 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9703.98 Da
• Isoelectric Point: 4.6768

>AT140476 WP_191904618.1 NZ_CP044230:569836-570090 [Streptococcus sp. LPB0220]
MEAVVYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVISKSEWDSIQETLRLAQNKELSDKVLRGMAEVRAGQVQLH
EIEG

Nucleotide

• Length: 255 bp

>AT140476 NZ_CP059281:c2726878-2726687 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• AlphaFold DB: I1ZKJ4