Detailed information of TA system TA140449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 2360305..2360938
• Replicon: chromosome
• Accession: NZ_CP044206
• Organism: Vibrio vulnificus strain Vv180806

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A347UQS4
• Locus tag: F6X00_RS11410
• Protein ID: WP_012600340.1
• Coordinates: 2360305..2360637 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F6X00_RS11415
• Protein ID: WP_017049537.1
• Coordinates: 2360624..2360938 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6X00_RS11355	2355347..2356351	+	1005	WP_182289715.1	DUF4917 family protein	-
F6X00_RS11360	2356529..2356759	-	231	Protein_2162	secretion protein	-
F6X00_RS11365	2356840..2356983	+	144	Protein_2163	DUF3265 domain-containing protein	-
F6X00_RS11370	2356993..2357550	+	558	WP_045596404.1	GNAT family N-acetyltransferase	-
F6X00_RS11375	2357547..2357675	+	129	WP_182290904.1	DUF3265 domain-containing protein	-
F6X00_RS11380	2358019..2358156	+	138	WP_182290907.1	DUF3265 domain-containing protein	-
F6X00_RS11385	2358159..2358593	+	435	WP_182289717.1	hypothetical protein	-
F6X00_RS11390	2358590..2358712	+	123	Protein_2168	DUF3265 domain-containing protein	-
F6X00_RS11395	2359528..2359689	+	162	WP_182289719.1	DUF3265 domain-containing protein	-
F6X00_RS11400	2359720..2360076	+	357	WP_020198239.1	hypothetical protein	-
F6X00_RS11405	2360077..2360202	+	126	WP_182290909.1	DUF3265 domain-containing protein	-
F6X00_RS11410	2360305..2360637	+	333	WP_012600340.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F6X00_RS11415	2360624..2360938	+	315	WP_017049537.1	DNA-binding transcriptional regulator	Antitoxin
F6X00_RS11420	2360935..2361045	+	111	WP_072611830.1	DUF3265 domain-containing protein	-
F6X00_RS11425	2361009..2361529	+	521	Protein_2175	hypothetical protein	-
F6X00_RS11430	2361685..2362116	+	432	WP_101957106.1	hypothetical protein	-
F6X00_RS11435	2362098..2362242	+	145	Protein_2177	DUF3265 domain-containing protein	-
F6X00_RS11440	2362585..2362728	+	144	WP_182290911.1	DUF3265 domain-containing protein	-
F6X00_RS11445	2362725..2363078	+	354	WP_042764103.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
F6X00_RS11450	2363174..2363623	+	450	WP_182290912.1	hypothetical protein	-
F6X00_RS11455	2363601..2363750	+	150	Protein_2181	DUF3265 domain-containing protein	-
F6X00_RS11460	2363766..2364332	+	567	WP_085761093.1	hypothetical protein	-
F6X00_RS11465	2364498..2364866	+	369	WP_011080320.1	VOC family protein	-
F6X00_RS11470	2364881..2364991	+	111	WP_080553496.1	DUF3265 domain-containing protein	-
F6X00_RS11475	2365029..2365610	+	582	WP_061897723.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	2268354..2394564	126210
	-	inside	Integrative and Conjugative Element	-	flp1 / VP1611	2163050..2680424	517374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-104)

Antitoxin

• Antitoxin MqsA/HigA-2 (24-101)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12906.76 Da
• Isoelectric Point: 10.0960

>T140449 WP_012600340.1 NZ_CP044206:2360305-2360637 [Vibrio vulnificus]
MKSVFVESTIFEKYRNEYLSDDEFRLFQAELMSNPKQGDVIQGTGGLRKIRVASKGKGKRGGSRVIYYFLDEKRRFYLLT
IYGKNEMSDLTADQKKQLKAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T140449 NZ_CP059279:3119144-3119518 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTACTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11704.17 Da
• Isoelectric Point: 6.3290

>AT140449 WP_017049537.1 NZ_CP044206:2360624-2360938 [Vibrio vulnificus]
MSNRDLFAELSSALVEAKEHSEGKLTLKTHQVNDISELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSAP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT140449 NZ_CP059279:3118687-3119055 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCAGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A347UQS4