Detailed information of TA system TA140414

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2366176..2366401
• Replicon: chromosome
• Accession: NZ_CP044158
• Organism: Shigella flexneri strain AR-0423

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: F4V35_RS12475
• Protein ID: WP_000813254.1
• Coordinates: 2366176..2366331 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2366343..2366401 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V35_RS12410	2361488..2361838	-	351	WP_005049241.1	IS66 family insertion sequence element accessory protein TnpB	-
F4V35_RS12415	2361835..2362509	-	675	WP_004967157.1	IS66-like element accessory protein TnpA	-
F4V35_RS12420	2362608..2362823	-	216	WP_000839572.1	class II holin family protein	-
F4V35_RS12450	2363619..2364307	-	689	Protein_2400	bacteriophage antitermination protein Q	-
F4V35_RS12455	2364304..2364669	-	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
F4V35_RS12460	2364670..2365728	-	1059	WP_001265248.1	DUF968 domain-containing protein	-
F4V35_RS12465	2365730..2366008	-	279	WP_011069426.1	hypothetical protein	-
F4V35_RS12475	2366176..2366331	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2366343..2366401	+	59	-	-	Antitoxin
F4V35_RS12490	2366983..2367399	-	417	WP_005069274.1	hypothetical protein	-
F4V35_RS12495	2367426..2367566	+	141	Protein_2406	DUF4224 domain-containing protein	-
F4V35_RS12500	2367566..2368616	+	1051	Protein_2407	tyrosine-type recombinase/integrase	-
F4V35_RS12510	2368827..2369624	+	798	WP_001325918.1	DgsA anti-repressor MtfA	-
F4V35_RS12520	2369962..2371224	+	1263	Protein_2409	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	2333549..2373991	40442

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T140414 WP_000813254.1 NZ_CP044158:c2366331-2366176 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T140414 NZ_CP059156:c2068711-2068535 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT140414 NZ_CP044158:2366343-2366401 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin