Detailed information of TA system TA140403

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1690614..1690839
• Replicon: chromosome
• Accession: NZ_CP044158
• Organism: Shigella flexneri strain AR-0423

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: F4V35_RS08685
• Protein ID: WP_000813254.1
• Coordinates: 1690684..1690839 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1690614..1690672 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V35_RS25670	1686613..1686782	+	170	Protein_1671	hypothetical protein	-
F4V35_RS08650	1686928..1687674	+	747	WP_000788996.1	ATP-binding protein	-
F4V35_RS08655	1687689..1688111	+	423	WP_001118168.1	DUF977 family protein	-
F4V35_RS08660	1688169..1688525	+	357	WP_005048249.1	hypothetical protein	-
F4V35_RS08665	1688618..1688835	+	218	Protein_1675	DUF4014 family protein	-
F4V35_RS08670	1688837..1689202	+	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
F4V35_RS08675	1689199..1689864	+	666	WP_000208062.1	hypothetical protein	-
F4V35_RS08680	1689864..1690229	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	1690614..1690672	-	59	-	-	Antitoxin
F4V35_RS08685	1690684..1690839	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
F4V35_RS08700	1692176..1692775	+	600	WP_000940329.1	DUF1367 family protein	-
F4V35_RS08705	1692775..1693065	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
F4V35_RS08710	1693062..1693615	+	554	Protein_1683	DUF1133 family protein	-
F4V35_RS08715	1693768..1693941	+	174	WP_000504450.1	hypothetical protein	-
F4V35_RS08720	1694003..1695142	+	1140	WP_000088354.1	IS3 family transposase	-
F4V35_RS08725	1695213..1695695	+	483	WP_000068107.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	1682698..1744550	61852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T140403 WP_000813254.1 NZ_CP044158:1690684-1690839 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T140403 NZ_CP059155:c2203453-2203091 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin

Nucleotide

• Length: 59 bp

>AT140403 NZ_CP044158:c1690672-1690614 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin