Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 672386..673004 Replicon chromosome
Accession NZ_CP044158
Organism Shigella flexneri strain AR-0423

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag F4V35_RS03345 Protein ID WP_001291435.1
Coordinates 672386..672604 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag F4V35_RS03350 Protein ID WP_000344797.1
Coordinates 672630..673004 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V35_RS03315 (668278) 668278..668589 - 312 WP_000409911.1 MGMT family protein -
F4V35_RS03325 (668968) 668968..669321 + 354 WP_000878140.1 DUF1428 domain-containing protein -
F4V35_RS03330 (669363) 669363..670913 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
F4V35_RS03335 (671077) 671077..671547 - 471 WP_000136192.1 YlaC family protein -
F4V35_RS03340 (671663) 671663..672214 - 552 Protein_656 maltose O-acetyltransferase -
F4V35_RS03345 (672386) 672386..672604 - 219 WP_001291435.1 HHA domain-containing protein Toxin
F4V35_RS03350 (672630) 672630..673004 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
F4V35_RS03355 (673550) 673550..676699 - 3150 WP_001132484.1 efflux RND transporter permease AcrB -
F4V35_RS03360 (676722) 676722..677915 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 667076..668248 1172


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T140399 WP_001291435.1 NZ_CP044158:c672604-672386 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T140399 NZ_CP059155:1935229-1935324 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT140399 WP_000344797.1 NZ_CP044158:c673004-672630 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT140399 NZ_CP059155:c1935409-1935352 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References