Detailed information of TA system TA140398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 635205..636049
• Replicon: chromosome
• Accession: NZ_CP044158
• Organism: Shigella flexneri strain AR-0423

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: F4V35_RS03145
• Protein ID: WP_014532172.1
• Coordinates: 635205..635666 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: F4V35_RS03155
• Protein ID: WP_005053053.1
• Coordinates: 635738..636049 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V35_RS03130 (630373)	630373..632103	-	1731	WP_000645013.1	hypothetical protein	-
F4V35_RS03135 (632156)	632156..634309	-	2154	WP_005060811.1	SEL1-like repeat protein	-
F4V35_RS03140 (634460)	634460..635157	+	698	WP_223368647.1	IS1 family transposase	-
F4V35_RS03145 (635205)	635205..635666	-	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
F4V35_RS03155 (635738)	635738..636049	-	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
F4V35_RS03160 (636233)	636233..637123	-	891	WP_000971346.1	heme o synthase	-
F4V35_RS03165 (637135)	637135..637464	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
F4V35_RS03170 (637464)	637464..638078	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
F4V35_RS03175 (638068)	638068..640059	-	1992	Protein_624	cytochrome o ubiquinol oxidase subunit I	-
F4V35_RS03180 (640081)	640081..640578	-	498	Protein_625	COX aromatic rich motif-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.004	flank	IS/Tn	-	-	634654..635157	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T140398 WP_014532172.1 NZ_CP044158:c635666-635205 [Shigella flexneri]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T140398 NZ_CP059155:c1601441-1601196 [Staphylococcus aureus]
ATGAACATTCAAGAAGCAACTAAGATAGCTACAAAAAATCTTGTCTCTATGACACGGAAAGATTGGAAAGAAAGTCATCG
AACTAAGATATTACCAACAAATGATAGTTTTTTACAATGCATCATTTCAAATAGCGATGGGACAAACCTTATCAGATATT
GGCAACCTTCAGCCGATGACCTCATGGCAAATGATTGGGAAGTTATAAACCCAACTAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT140398 WP_005053053.1 NZ_CP044158:c636049-635738 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT140398 NZ_CP059155:1600862-1601227 [Staphylococcus aureus]
ATGCCCTTGATATCTGATGAATTTGATACACTTACTAAAGACCAACAATATATCTTGTCCGTACTCTACAAAGATTATTT
AGAATGTGTAAAGTTAGGTTCGGTTAAATTAACCTGCAATAATTTTGGAAGTGCTAAAGATATACATACAAAGTATTTTC
AAAAACTACATTTCGAAGATGTAAAATACGATTTAAATAAACTTAAAAACTCTGGGTTCCTAAACGGCGTGTATGCTAGT
AACACTATTTATCATGTAACAATTTCAGACAAGACTGTTGTTTACTTTGAAAATGAGTTTAAAAACAATTTAAAAAGTAT
CATTGATAGCATTTCTAAAATTGCTTCAATAATTCCTGGTCTCTAG

Structures

Antitoxin

• AlphaFold DB: A5H0K0