Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 555257..555951 Replicon chromosome
Accession NZ_CP044158
Organism Shigella flexneri strain AR-0423

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag F4V35_RS02740 Protein ID WP_001263491.1
Coordinates 555553..555951 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag F4V35_RS02735 Protein ID WP_000554759.1
Coordinates 555257..555550 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V35_RS02715 (550896) 550896..551393 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
F4V35_RS02720 (551610) 551610..553322 - 1713 Protein_536 flagellar biosynthesis protein FlhA -
F4V35_RS02725 (553294) 553294..554079 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
F4V35_RS02730 (554150) 554150..555205 + 1056 WP_001226172.1 DNA polymerase IV -
F4V35_RS02735 (555257) 555257..555550 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
F4V35_RS02740 (555553) 555553..555951 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
F4V35_RS02745 (555961) 555961..556413 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
F4V35_RS02750 (556659) 556659..557702 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
F4V35_RS02755 (557818) 557818..558378 + 561 Protein_543 peptide chain release factor H -
F4V35_RS02760 (558435) 558435..559892 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
F4V35_RS02765 (560154) 560154..560612 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T140397 WP_001263491.1 NZ_CP044158:555553-555951 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T140397 NZ_CP059155:c1594254-1594003 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCCGTTATCAGTGCTAGTGCGTATAACGGTAATGATACAGAGGGATTACTAAA
AGAGATTGAGGACGTATATAAGAAAGCGCGAGCGTTTGATGAAATACTTGAAGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGGTCTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT140397 WP_000554759.1 NZ_CP044158:555257-555550 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT140397 NZ_CP059155:c1594013-1593831 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTACAAGTTATCTGACGGCTCTATTATCAGTCTCATAGACTTTGTTATTAAACCAATTCATTTAATCA
AGGAGGCACAGGAAAATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References