Detailed information of TA system TA140388

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39068..39321
• Replicon: plasmid pAR-0424-1
• Accession: NZ_CP044156
• Organism: Shigella flexneri strain AR-0424

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: F4V36_RS23750
• Protein ID: WP_001312851.1
• Coordinates: 39068..39217 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39262..39321 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V36_RS23710 (35090)	35090..35491	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
F4V36_RS23715 (35424)	35424..35681	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
F4V36_RS23720 (35774)	35774..36427	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
F4V36_RS25770 (36525)	36525..36665	-	141	WP_001333237.1	hypothetical protein	-
F4V36_RS23730 (37366)	37366..38223	-	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
F4V36_RS23735 (38216)	38216..38290	-	75	WP_001365705.1	RepA leader peptide Tap	-
F4V36_RS23745 (38535)	38535..38783	-	249	WP_000083839.1	replication regulatory protein RepA	-
F4V36_RS23750 (39068)	39068..39217	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (39262)	39262..39321	+	60	NuclAT_1	-	Antitoxin
- (39262)	39262..39321	+	60	NuclAT_1	-	Antitoxin
- (39262)	39262..39321	+	60	NuclAT_1	-	Antitoxin
- (39262)	39262..39321	+	60	NuclAT_1	-	Antitoxin
F4V36_RS23760 (39464)	39464..39937	-	474	WP_016240489.1	hypothetical protein	-
F4V36_RS23765 (40092)	40092..40682	-	591	WP_033807967.1	DUF2726 domain-containing protein	-
F4V36_RS23770 (40720)	40720..40929	-	210	WP_001298565.1	hemolysin expression modulator Hha	-
F4V36_RS23775 (40975)	40975..41436	-	462	WP_001233850.1	thermonuclease family protein	-
F4V36_RS23780 (41682)	41682..41894	-	213	WP_001301409.1	ANR family transcriptional regulator	-
F4V36_RS23785 (42026)	42026..42586	-	561	WP_033807966.1	fertility inhibition protein FinO	-
F4V36_RS23790 (42641)	42641..43387	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / sul1 / qacE / aadA5 / blaTEM-1B	-	1..79831	79831

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T140388 WP_001312851.1 NZ_CP044156:c39217-39068 [Shigella flexneri]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T140388 NZ_CP059130:c4684487-4683960 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT140388 NZ_CP044156:39262-39321 [Shigella flexneri]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin