Detailed information of TA system TA140382

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4250527..4250752
• Replicon: chromosome
• Accession: NZ_CP044155
• Organism: Shigella flexneri strain AR-0424

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: F4V36_RS21485
• Protein ID: WP_000813254.1
• Coordinates: 4250597..4250752 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	4250527..4250585 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V36_RS26275	4246525..4246694	+	170	Protein_4106	hypothetical protein	-
F4V36_RS21450	4246840..4247586	+	747	WP_000788996.1	ATP-binding protein	-
F4V36_RS21455	4247601..4248023	+	423	WP_001118168.1	DUF977 family protein	-
F4V36_RS21460	4248081..4248437	+	357	WP_005048249.1	hypothetical protein	-
F4V36_RS21465	4248530..4248748	+	219	WP_151106435.1	DUF4014 family protein	-
F4V36_RS21470	4248750..4249115	+	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
F4V36_RS21475	4249112..4249777	+	666	WP_000208062.1	hypothetical protein	-
F4V36_RS21480	4249777..4250142	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	4250527..4250585	-	59	-	-	Antitoxin
F4V36_RS21485	4250597..4250752	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
F4V36_RS21500	4252089..4252688	+	600	WP_000940329.1	DUF1367 family protein	-
F4V36_RS21505	4252688..4252978	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
F4V36_RS21510	4252975..4253529	+	555	WP_000640143.1	DUF1133 family protein	-
F4V36_RS21515	4253682..4253855	+	174	WP_000504450.1	hypothetical protein	-
F4V36_RS21520	4253917..4255056	+	1140	WP_151106437.1	IS3 family transposase	-
F4V36_RS21525	4255127..4255609	+	483	WP_000068107.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	4242610..4304777	62167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T140382 WP_000813254.1 NZ_CP044155:4250597-4250752 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T140382 NZ_CP059130:c3005530-3005276 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT140382 NZ_CP044155:c4250585-4250527 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin