140381

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3265236..3265854 Replicon chromosome
Accession NZ_CP044155
Organism Shigella flexneri strain AR-0424

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag F4V36_RS16370 Protein ID WP_001291435.1
Coordinates 3265236..3265454 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag F4V36_RS16375 Protein ID WP_000344797.1
Coordinates 3265480..3265854 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V36_RS16340 (3261128) 3261128..3261439 - 312 WP_000409911.1 MGMT family protein -
F4V36_RS16350 (3261818) 3261818..3262171 + 354 WP_000878140.1 DUF1428 domain-containing protein -
F4V36_RS16355 (3262213) 3262213..3263763 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
F4V36_RS16360 (3263927) 3263927..3264397 - 471 WP_000136192.1 YlaC family protein -
F4V36_RS16365 (3264513) 3264513..3265064 - 552 Protein_3129 maltose O-acetyltransferase -
F4V36_RS16370 (3265236) 3265236..3265454 - 219 WP_001291435.1 HHA domain-containing protein Toxin
F4V36_RS16375 (3265480) 3265480..3265854 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
F4V36_RS16380 (3266400) 3266400..3269549 - 3150 WP_001132484.1 efflux RND transporter permease AcrB -
F4V36_RS16385 (3269572) 3269572..3270765 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3259770..3261098 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T140381 WP_001291435.1 NZ_CP044155:c3265454-3265236 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T140381 NZ_CP059130:2994369-2994743 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT140381 WP_000344797.1 NZ_CP044155:c3265854-3265480 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT140381 NZ_CP059130:2993912-2994280 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References