Detailed information of TA system TA140380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3228054..3228898
• Replicon: chromosome
• Accession: NZ_CP044155
• Organism: Shigella flexneri strain AR-0424

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: F4V36_RS16170
• Protein ID: WP_014532172.1
• Coordinates: 3228054..3228515 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: F4V36_RS16180
• Protein ID: WP_005053053.1
• Coordinates: 3228587..3228898 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V36_RS16155 (3223222)	3223222..3224952	-	1731	WP_000645013.1	hypothetical protein	-
F4V36_RS16160 (3225005)	3225005..3227158	-	2154	WP_005060811.1	SEL1-like repeat protein	-
F4V36_RS16165 (3227309)	3227309..3228006	+	698	WP_223368647.1	IS1 family transposase	-
F4V36_RS16170 (3228054)	3228054..3228515	-	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
F4V36_RS16180 (3228587)	3228587..3228898	-	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
F4V36_RS16185 (3229082)	3229082..3229972	-	891	WP_000971346.1	heme o synthase	-
F4V36_RS16190 (3229984)	3229984..3230313	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
F4V36_RS16195 (3230313)	3230313..3230927	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
F4V36_RS16200 (3230917)	3230917..3232908	-	1992	Protein_3097	cytochrome o ubiquinol oxidase subunit I	-
F4V36_RS16205 (3232930)	3232930..3233427	-	498	Protein_3098	COX aromatic rich motif-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.004	flank	IS/Tn	-	-	3227503..3228006	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T140380 WP_014532172.1 NZ_CP044155:c3228515-3228054 [Shigella flexneri]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T140380 NZ_CP044155:c3228515-3228054 [Shigella flexneri]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT140380 WP_005053053.1 NZ_CP044155:c3228898-3228587 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT140380 NZ_CP044155:c3228898-3228587 [Shigella flexneri]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0