Detailed information of TA system TA140373

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 321979..322204
• Replicon: chromosome
• Accession: NZ_CP044155
• Organism: Shigella flexneri strain AR-0424

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: F4V36_RS01825
• Protein ID: WP_000813254.1
• Coordinates: 321979..322134 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	322146..322204 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V36_RS01760	317291..317641	-	351	WP_005049241.1	IS66 family insertion sequence element accessory protein TnpB	-
F4V36_RS01765	317638..318312	-	675	WP_004967157.1	IS66-like element accessory protein TnpA	-
F4V36_RS01770	318411..318626	-	216	WP_000839572.1	class II holin family protein	-
F4V36_RS01800	319422..320110	-	689	Protein_338	bacteriophage antitermination protein Q	-
F4V36_RS01805	320107..320472	-	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
F4V36_RS01810	320473..321531	-	1059	WP_001265248.1	DUF968 domain-containing protein	-
F4V36_RS01815	321533..321811	-	279	WP_011069426.1	hypothetical protein	-
F4V36_RS01825	321979..322134	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	322146..322204	+	59	-	-	Antitoxin
F4V36_RS01840	322785..323201	-	417	WP_005069274.1	hypothetical protein	-
F4V36_RS01845	323228..323368	+	141	Protein_344	DUF4224 domain-containing protein	-
F4V36_RS01850	323368..324418	+	1051	Protein_345	tyrosine-type recombinase/integrase	-
F4V36_RS01860	324629..325426	+	798	WP_001325918.1	DgsA anti-repressor MtfA	-
F4V36_RS01870	325764..327026	+	1263	Protein_347	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	306935..329543	22608

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T140373 WP_000813254.1 NZ_CP044155:c322134-321979 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T140373 NZ_CP059130:703074-703361 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT140373 NZ_CP044155:322146-322204 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin