Detailed information of TA system TA140358

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4397672..4397897
• Replicon: chromosome
• Accession: NZ_CP044152
• Organism: Shigella flexneri strain AR-0425

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: F4V37_RS22330
• Protein ID: WP_000813254.1
• Coordinates: 4397742..4397897 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	4397672..4397730 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V37_RS22285	4392850..4394112	-	1263	Protein_4285	tyrosine-type recombinase/integrase	-
F4V37_RS22295	4394450..4395247	-	798	WP_001325918.1	DgsA anti-repressor MtfA	-
F4V37_RS22305	4395458..4396507	-	1050	Protein_4287	tyrosine-type recombinase/integrase	-
F4V37_RS22310	4396507..4396647	-	141	Protein_4288	DUF4224 domain-containing protein	-
F4V37_RS22315	4396674..4397090	+	417	WP_005069274.1	hypothetical protein	-
-	4397672..4397730	-	59	-	-	Antitoxin
F4V37_RS22330	4397742..4397897	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
F4V37_RS22340	4398065..4398343	+	279	WP_011069426.1	hypothetical protein	-
F4V37_RS22345	4398345..4399403	+	1059	WP_001265248.1	DUF968 domain-containing protein	-
F4V37_RS22350	4399404..4399769	+	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
F4V37_RS22355	4399766..4400454	+	689	Protein_4294	bacteriophage antitermination protein Q	-
F4V37_RS22385	4401250..4401465	+	216	WP_000839572.1	class II holin family protein	-
F4V37_RS22390	4401564..4402238	+	675	WP_004967157.1	IS66-like element accessory protein TnpA	-
F4V37_RS22395	4402235..4402585	+	351	WP_005049241.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	4353454..4429746	76292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T140358 WP_000813254.1 NZ_CP044152:4397742-4397897 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T140358 NZ_CP059125:c3648869-3648534 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 59 bp

>AT140358 NZ_CP044152:c4397730-4397672 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin