Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3245029..3245756 Replicon chromosome
Accession NZ_CP044152
Organism Shigella flexneri strain AR-0425

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag F4V37_RS16545 Protein ID WP_000550189.1
Coordinates 3245029..3245343 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag F4V37_RS16550 Protein ID WP_000560266.1
Coordinates 3245340..3245756 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V37_RS16525 (3241174) 3241174..3242172 - 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -
F4V37_RS16530 (3242251) 3242251..3242943 - 693 WP_000942538.1 vancomycin high temperature exclusion protein -
F4V37_RS16535 (3243020) 3243020..3243523 - 504 WP_005050988.1 M48 family metallopeptidase -
F4V37_RS16540 (3243608) 3243608..3244744 + 1137 WP_000018656.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
F4V37_RS16545 (3245029) 3245029..3245343 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
F4V37_RS16550 (3245340) 3245340..3245756 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
F4V37_RS16555 (3245801) 3245801..3247819 - 2019 WP_000121487.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
F4V37_RS16560 (3248045) 3248045..3250396 - 2352 WP_000695506.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T140355 WP_000550189.1 NZ_CP044152:3245029-3245343 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T140355 NZ_CP059125:c2812989-2812738 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT140355 WP_000560266.1 NZ_CP044152:3245340-3245756 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT140355 NZ_CP059125:c2812741-2812487 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References