Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1572210..1572904 Replicon chromosome
Accession NZ_CP044152
Organism Shigella flexneri strain AR-0425

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag F4V37_RS08335 Protein ID WP_001263491.1
Coordinates 1572210..1572608 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag F4V37_RS08340 Protein ID WP_000554759.1
Coordinates 1572611..1572904 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V37_RS08310 (1567549) 1567549..1568007 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
F4V37_RS08315 (1568269) 1568269..1569726 + 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
F4V37_RS08320 (1569783) 1569783..1570343 - 561 Protein_1605 peptide chain release factor H -
F4V37_RS08325 (1570459) 1570459..1571502 - 1044 WP_005053205.1 RNA ligase RtcB family protein -
F4V37_RS08330 (1571748) 1571748..1572200 - 453 WP_001059860.1 GNAT family N-acetyltransferase -
F4V37_RS08335 (1572210) 1572210..1572608 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
F4V37_RS08340 (1572611) 1572611..1572904 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
F4V37_RS08345 (1572956) 1572956..1574011 - 1056 WP_001226172.1 DNA polymerase IV -
F4V37_RS08350 (1574082) 1574082..1574867 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
F4V37_RS08355 (1574839) 1574839..1576551 + 1713 Protein_1612 flagellar biosynthesis protein FlhA -
F4V37_RS08360 (1576768) 1576768..1577265 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrII / gtrB / gtrA 1538335..1577995 39660


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T140351 WP_001263491.1 NZ_CP044152:c1572608-1572210 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T140351 NZ_CP059125:c1912667-1912560 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT140351 WP_000554759.1 NZ_CP044152:c1572904-1572611 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT140351 NZ_CP059125:1912714-1912780 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References