Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1455161..1455779 Replicon chromosome
Accession NZ_CP044152
Organism Shigella flexneri strain AR-0425

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag F4V37_RS07730 Protein ID WP_001291435.1
Coordinates 1455561..1455779 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag F4V37_RS07725 Protein ID WP_000344797.1
Coordinates 1455161..1455535 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V37_RS07715 (1450249) 1450249..1451442 + 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
F4V37_RS07720 (1451465) 1451465..1454614 + 3150 WP_001132484.1 efflux RND transporter permease AcrB -
F4V37_RS07725 (1455161) 1455161..1455535 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
F4V37_RS07730 (1455561) 1455561..1455779 + 219 WP_001291435.1 HHA domain-containing protein Toxin
F4V37_RS07735 (1455951) 1455951..1456502 + 552 Protein_1492 maltose O-acetyltransferase -
F4V37_RS07740 (1456618) 1456618..1457088 + 471 WP_000136192.1 YlaC family protein -
F4V37_RS07745 (1457252) 1457252..1458802 + 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
F4V37_RS07750 (1458844) 1458844..1459197 - 354 WP_000878140.1 DUF1428 domain-containing protein -
F4V37_RS07760 (1459576) 1459576..1459887 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1459917..1461245 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T140349 WP_001291435.1 NZ_CP044152:1455561-1455779 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T140349 NZ_CP059125:c1108428-1108210 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT140349 WP_000344797.1 NZ_CP044152:1455161-1455535 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT140349 NZ_CP059125:c1108828-1108454 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References